草魚呼腸孤病毒人工感草魚的條件篩選和病理學(xué)研究.pdf

1、四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文草魚呼腸孤病毒人工感草魚的條件篩選和病理學(xué)研究姓名：肖雪申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：顏其貴郭萬柱20070601TheboltingconditionsofGrassCarpHemorrhageDiseasebyartificialinfectionandPathologyAnalysisXiaoXueDirectedbyAssociateProfessorYanQigui(AnimalBiote

2、chnologyCenterofSichuanAgriculturalUniversityYaan，SichuanChina625014)ABSTRACTC】IassCarpReovirus(OCRV)isonememberofAquareovinmintheReoviridaefamilywhichpossessesthemoststrongtoxicityandseriouslythreatensthefishindus姆offre

3、shwatercultivationinOUrcountry1990s，OurcountryhascompletedtheGCRVentiregenesequenceanalysisoninternationalforthefirsttime，andwhatismoie，GCRVisthoroughlystudiedaquareovirusuptonOWinOUrworldDesignsGCRV873artificialtoinfect

4、grasscarpandestablishespathologicalmodelThepurchasedvirusGCRV873hasbeenconservedin一70℃sin∞1997，t吲cityofvill∞r(nóng)educesthescopetobebiggervirushandsdownfromgenerationtogenerationa廟盯thecell，thetoxicityslightlyrises越兩ll’itsTCID

5、50is103881／100uhVhusculturedincellsc∞notCaUSethegrasscarppathogenesisatfirstItappearstheobviouspathologicalchangewhen汛handsdowninthegrasscarpafter5generationmainlyeaus∞theredmusclepathologicalchangeWecarryontheofficialex

6、perimentbyusingmanufacturesicknessVel]tolnwhichmadebythegrasscarpinternalorg∞shastheobvioussymptorllGrasscarpwithinfectionhastheclinicalsymptomarisesin4thdays，achievesthedeathpeakin7thdays，enterstransfersthedateofreturni

7、ntheatLcwardsInfectiousgrasscarpdisplayscharacteristic“ExcitableHemorrhageDisease“whichblushe、hyperemia、hemorrhageshowsinskills、muscle、fishfinbaseFromHistopathologyobservation，itcarlbe8eenfattyorvacuolardegenerationinliv

8、erceils，vacool暫degenerationhappensintherenaltubularepithelialcellandthecardiacmusclecells；Thekidneybloodpipewallisdestroyedthebloodcorpuscleleakoff；Spleencellbureaustovenecrosis；Cardicmuscleandskeletalmusclefiberwaxtypeh

9、emorrhageandnecrosisUlwasturctorally，theviralgranulemainlyexiststOthekidney，otherorganshavenotseenthereforethekidneypathologicalchangeismostseriousThemitochondriaofthecardiacmusclecells，thefivercellsandtherenaltubularepi