桂花切花花瓣衰老過(guò)程中的蛋白質(zhì)變化分析.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文桂花切花花瓣衰老過(guò)程中的蛋白質(zhì)變化分析姓名：徐幸福申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：園林植物與觀賞園藝指導(dǎo)教師：王彩云20030501StudiesontheProteinChangesdunngPetalSenescenceofAbstractOsmanthus(Osmanthus步49r4砼sLout)isoneofthetraditionalornamentalflowerandiswildelyusedingard

2、ensbecauseofitsspecificfragranceWiththebreedoflargeflowervarieties，osmanthushasbecomeakindofcutflowerresourcewitllgooddevelopingprospectButosmanthusomamentalperiodisshortenedbyitsshortflorescenceandthislimitsitsdevelopme

3、ntascutflowerNowadays，controllingthesenescenceofpetaltoprolongflorescencebygeneticengineeringhasobtainedgreatdevelopmentnlisexperimentestablishedtheSDSPAGEexperimentalsystemforproteinsofpetalfromosmanthus(Ofragrans‘Houba

4、nJingui’)，andstudiedthesenescenceofpetalofosmamhusinproteinlevel，whichwillbringsomeinformationforosmanthusgeneticbreedThemainresultsinthisexperimentwereshownaSfollows：1Thedeterminationofproteinextractionmethodforelectrop

5、horesisBasedonstudyingandcomparingothermethods，animprovedacetonesedimentmethodwasachieyedBythismethod，theresultsofSDSPAGEhadclearproteinbandswi也Iittlebackground，2EstablishmentofSDSPAGEexperimentalsystemforpetalproteinsof

6、osmanthusAftercomparingtheresultsofelectrophoresis，theconditionsofelectrophoresisweremodified：concentrationofrunninggelWas11％witlIlengthof910cmtheconcentrationofstackinggelWas39％with152Ocmtheamountofaddedproteinsampleswe

7、re10lalintheventsof1025lmm3thenmningtimeWas25hoursorsoandgelswerestained、^ritlltheprotocolselectedfromseveralstainingmethodsTheproteinsbandswereclearanditsbackgroundwaslowintheresultsofelectrophoresisBasedonthis，someprel

8、iminarystudyon2DimansionalelectrophoresisforproteinsofpetalWascarriedout3nleresultsofSDS—PAGEofproteinsduringsenescentpetalofcutosmanthusnleexperimentanalysisshowed：398kDand275kDpeptideswerestablefrombeginningtotheend376

9、kDpeptidedecreasedgr刪lyand631kD、575kD、515kDpeptidesappearedandincreasedwiththesenescenceofpetal515kD、323kDpaptideschangedafterethyleneandABAtreatmentThisimpliesthatthereissomerelationshipbetweenchangesofpeptidesandsenesc