轉染BRMS1基因對胃癌SGC7901細胞增殖及體外侵襲力的影響.pdf

1、南昌大學醫(yī)學院碩士學位論文轉染BRMS1基因對胃癌SGC7901細胞增殖及體外侵襲力的影響姓名：向德雨申請學位級別：碩士專業(yè)：外科學（普外）指導教師：揭志剛20080601AbstractABSTRACTPurpose：WilleukaryoticexpressionvectorpcDNA3I(一)B／myeBRMS1tmnsfectedintoSGC7901gastriccancel“cells，BRMSlobservedinvitr

2、ogeneSGC7901cellproliferationandinvasioncapacityofMethod=ConventionalcultureofgastriccancercellsSGC7901，RTPCRmethodtodetectSGC7901gastriccancel“cellsBRMS1mRNAexpression；WithliposomesLIPOFECTAMINE2000willpcDNA31／mychis()B

3、andpcDNA31／myeBRMSItransfectedintogastricCanCercellsSGC7901，48hourSaftertransfectionwith300ug／mloftheG418pressurescreening28days，Resistancetobeformedafterthecloningofhumanbeingswim96cellcultureplatelimiteddilutionpickfro

4、mmonoclonal，Monoclonalwilluse170ug／mloftheG418tomaintainandexpandscreeningtraining；AfterthetrainWasamplifiedbyRTPCRandWesternBlotmethodscomparebeforeandafterthetransferBRMS1changesingeneexpressionFromtransfeetedBRMS1them

5、ostobviouschangesingeneexpressioninthecellcloningmethoddrawnbyMTTcellgrowthcurve，Boydenchamber、)l，imimprovedlinesofcellsinvitroinvasionandmetastasisexperimentResults：SGC7901gastricCanCercellsingeneexpressionwithoutBRMS1；

6、TransfectioneukaryoticexpressionvectorpcDNA31()B／mye—BRMSlGroupreeeiyesthreemonoclonal，WerenamedasSGC7901R1，SGC7901R2，SGC7901R3；TransfectedwithemptyvectorpeDNA31／myehis()BgroupreceivedtwomonoclonM，WerenamedasSGC7901一VI，S

7、GC7901一V2；ByRTPCRtestaftertransfectionBRMS1mRNAexpressionchanges；SeetransferofeukaryoticexpressionvectorofonlythreemonoclonalSGC7901R3hasBRMS1geneexpression，TransferofPgroupoftwomonoclonalnoBRMS1geneexpressio；WillSGC7901

8、，SGC7901V1，SGC7901一R3cellsweredrawnbyMTTmethodofgrowthcurve，Theresultsshowthat：BRMS1genedoesnotaffectgastricCanCercellproliferationSGC7901；Boydellchamberwithimprovedlinesofcellsinvitroinvasionandmetastasisexperiment，Ther