S100A10和H11基因在小鼠胚胎植入中的功能研究.pdf

1、湖南農(nóng)業(yè)大學碩士學位論文S100A10和H11基因在小鼠胚胎植入中的功能研究姓名：陳志強申請學位級別：碩士專業(yè)：基礎獸醫(yī)學指導教師：章懷云王雁玲20050501StudiesonSIOOAIOandHIIGenesFunctioninMouseEmbryonicImpIantatj011AbstractEmbryonicimplantationisatemporallyandspatiallyrestrictedprocess，whic

2、hinitiatesatarestrictedstageconsideredasthe“implantationwindow’Meanwhile，theadhesionandinvasionoftheembryointomaternalendometriumOccursatthespecificregionofuterusthatisnamedasthe“implantationsite”Asuccessfulimplantationi

3、nvolvesacoordinatedinteractionbetweenthematernalendometriumandtheblastocyst，andispreciselyregulatedbyvariousfactorsderivedfromboththeembryoandthematernaluterusThisisahottopicinreproductivebiologyinpresentPreviouslywefoun

4、dthatSIOOAIOandHllgenesweresignificantlyupregulatedinrhesusmonkey(Macacamulatta)implantationsiteatthebeginningofpregnancybyusingsuppressionsubtractivehybridization(ssH)method，indicatingthatS100A10andH11mayfunctioninthepr

5、ocessofembryoimplantationButtheirfunctioninreproductivesystemremainsunclearInthepresentstudysemi—quantitativereversetranscriptasepolymerasechainreactionandinsituhybridizationaleusedtoinvestigatethetissuespecificexpressio

6、nofSIOOAIOandHligenesandtheexpressionpatternsofSlOOAlOandHllgenesintheuteriduringtheestrouscycleandpregnancyMeanwhiletheregulationofSIOOAl0andHjjgenesexpressionbysexsteroidhormones(E2andP4)wasstudiedinovariectomizedmiceT

7、heresultsshowedthat：(1)SIOOAIOand劇，genescouldbedetectedinvariouskindsofmicetissues，withrelativelyhighexpressioninfemalereproductivetractsincludingovaryanduterus(2)Duringpregnancy，theexpressionofSlOOAlOandHIIgenesintheute

8、riissignificantlyupregulatedonthe4thdaywhenembryonicimplantationinitiatesThetranscriptwasextensivelylocalizedin肌dometrialluminalepitheliumcellsaswellasstromalcellsattheimplantationsitewhilealmostnondetectableattheinterim

9、plantationsite(3)Duringtheestrouscycle，expressionofSIOOAIOisup—regulatedintheuteriatproestrusandesffusTheexpressionlevelofHllgenemaintainedatahighlevelduringthewholeestruscycle，onlydecreasedintheproestrus(4)Fromgestation