重組大腸桿菌BL21（DE3）-rbLF-N高密度培養(yǎng)條件優(yōu)化.pdf

1、中國農(nóng)業(yè)大學碩士學位論文重組大腸桿菌BL21（DE3）rbLFN高密度培養(yǎng)條件優(yōu)化姓名：林少華申請學位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工與貯藏工程指導教師：葛克山任發(fā)政20070601AbstractLactofcrrcin，releasedfromtheNterminusoflactoferrinbypepsincleavageatacidicconditionisafragmaaofpolypeptideLactoferrcinhmmany

2、uniquebiologicalfunctions，inwhichtheantimicrobialactivityhasbeenextensive掃investigatedDuetolimitedresourcgandhighcostofthematralbovinelactoferrcingenecloningDNArecombinantandhighcelldensityculturetechnologieSmeapplied，re

3、ducingthecosLimprovingtheefficiencyandcreatingmorebenefitsInthepaper，thefermentationculturemediumsofEcoilBL2l(DE3)／toLFNhavebeenoptimizedTheoptimummediumcompositionswereasfollows(ing，L)：trypteml6，yeastexlrnctlO，glucose10

4、，NaCI5，citricacid3，NaHlP04‘2H2028，Na2HP04‘12H2065，Ko腫04‘3H204I，(hat4)2S0410，Ntt4C10I，MgS04‘7H20I13，ZnSq711200028，CaClz2H20004，MnS04H20O0l，AICl36H20001，CroCi2H200001，H3130200005，F(xiàn)eS047E【2000054mfinalharvestdrycellweightof

5、EcoltBL21(DE3)／rbLFNfermentedintheoptimumculturemediumsWag178timesasmanyastha￡beforeoptimizationTheoptimuminductionconditionWagthecombinationofadditionofO1mMIPTGwith20g，LlactoseFedbatchcultureprocessofEcoilBL21(DE3)／rbLF

6、NWascarriedouttoproducerecombinantbovinelactofcrrinNpolypeptide(枷—N)ina19LautoconlrolbioengineeringfcrmentorSeveralfeedingstrategies，includingbatchfermentation，pH—statfeeding，exponentialfeedingandcombinat／onofexponential

7、feedingandpHstatfeeding，WeIecomparedtoobtainthevolumetricprodnctiviti％Finally，thecombinedstrategyWasadoptedandhigherconcentrationl印to81359／L(bydrycellweight，DCW)ofbrothbiomassand5259幾rbLFNwereobtainedTheaccumulationoface

8、tateOVCY259／L，whichusual】yoccurredduringtheprocessofhighdensitycultureofEscherichia蒯andespeciallyintheinductionstageWaSavoidedThcplasmidstabilityWasmaintainedathighlevelsKeyword：Lactofetrcin，RecombinantEcoliBL21(DE3)，F(xiàn)er