玉米大斑病菌原生質(zhì)體制備、再生及根癌農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化研究.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)碩士學(xué)位論文玉米大斑病菌原生質(zhì)體制備、再生及根癌農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化研究姓名：宋慶濤申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物病理學(xué)指導(dǎo)教師：張國(guó)珍2002.6.1AbstractTheeffectofthemediaPDA，CM，BM，BCM，20“12and25“C，lightoffluorescentdark12hNuVandbyturns12hlightoffluorescentonthecondialproductionof肪砌fh

2、ⅢfmfcⅢ’0109_8werestudiedTheresultsindicatedthattheoptimumconditionforcondialproductionwasthatthestrainilluminatedwith12hNOVandbyturns12hlightoffluorescentwasculturedonBCMat20“Cfor20days，andthetotalamountofsporescouldbeup

3、to41610。perpetridishToobminalargeamountofprotoplastsfortransformationtheeffectsoftheageofhyphae，liquidmediaenzymesystems，timeofdigestionandstabilizeroiltheprotoplastpreparationandtheeffectofstabilizerontheprotoplastsrege

4、nerationwerestudiedTheresultsindicatedthattheoptimumconditionf醅preparingprotoplastswastheeondiaculturedinliquidFriesmediumfor24herlz“ymemixtureof1％Lywallzyrne。1％Snailaseandl％Drislase№cdtodigestthehyphaefor5h，07MKCIused∞t

5、heosmoticstabilizerwecanobtain296X106per059wetmycelia：06MsucroseastheosmoticstabiliZerforthepmtoplastregeneration，thepercentageofregenerationcouldbeupto753％ThecondiaofExserohilumttwdctlm01098weretransformedbytheAgrobacte

6、riwntumefaciensmediatedintegrationThetesultsshowedthattheprimarytransformantsappearedin57daysafterthecoculturedcondiaweretransferredtotheselectivemediumcontaininghygromycin1001tg／m1Thehighestnumberoftheprimarytransforman

7、tswere75in50XlOscondia187primarytransfomantswereobtained111eygrewslowlywhensubculturedontheselectivemediumcontainingthehygmmycinlOOpg／m1NineofthemshowedtheabnormalgrowthinPDAcontainingnohygrimy4inThirtyprimarytansfomants