24336.擬南芥紫色酸性磷酸酶atpap2和atpap9對磷脅迫的響應(yīng)

1、分類號Q75學(xué)校代碼10129UDC577學(xué)號08210039擬南芥紫色酸性磷酸酶AtPAP2和AtPAP9對磷脅迫的響應(yīng)ResponseoftheTwoArabidopsisPurpleAcidPhosphataseAtPAP2AtPAP9toPhosphusStress申請人：張娜學(xué)科門類：理學(xué)學(xué)科專業(yè)：生物化學(xué)與分子生物學(xué)研究方向：植物生物化學(xué)與分子生物學(xué)指導(dǎo)教師：王瑞剛教授叢靖宇講師論文提交日期：二〇一一年五月Responseo

2、ftheTwoArabidopsisPurpleAcidPhosphataseAtPAP2AtPAP9toPhosphusStressAbstractPhosphusplaysacriticalroleinplantgrowthdevelopment.Howeverplantsareoftenexposedtophosphusvacancyduetothelowconcentrationofutilizablephosphusthatp

3、lantscouldabsb.Whileoverapplicationofphosphusoftenresultsinplantphosphism.Underlowphosphusstressplantcouldadapttotheenvironmentbysecretingpurpleacidphosphatase(PAP)intosoilhydrolyzetheganophosphuscompoundsrichinsoil.Inte

4、nsivestudiesshowedthatmanymembersinArabidopsisPAPproteinfamilywhichcontains29membersareinvolvedinphosphusnutrition.InthisdissertationfunctionoftwooftheAtPAPsAtPAP2AtPAP9werestudiedusingtheircrespondingTDNAionmutants.Ther

5、esultsshowed:1.Atpap2Atpap9homozygousknockoutmutantswereidentifiedconfirmedbyPCRsemiquantitativeRTPCR.2.UnderphosphusstressAtpap2mutantshowedamesinsitivephenotypethanwildtypewhileAtpap9mutanthadnoobviousdifferencecompare

6、dwithwildtype.3.Atpap29doublemutantwasobtainedbycrossingthesinglemutantsAtpap2Atpap9.4.UnderphosphusstressthedoublemutantshowedameseveresressedphenotypethanAtpap2singlemutantsuggestingthatAtPAP2AtPAP9arefunctionallyredun

7、dantinresponsetophosphusstressinArabidopsis.KeyWds:ArabidopsisthalianaPurpleacidphosphataseMutantAtPAP2AtPAP9PhosphusstressDirectedby：Profess:WANGRuigangLecturer:CONGJingyuApplicantfMasterdegree：ZHANGNa(Biochemistry&Mole