雞與鵪鶉屬間雜交早期胚胎中PGCs的觀察及Aromatase，ER基因的表達分析.pdf

1、石河子大學碩士學位論文雞與鵪鶉屬間雜交早期胚胎中PGCs的觀察及Aromatase，ER基因的表達分析姓名：薛安永申請學位級別：碩士專業(yè)：動物遺傳育種與繁殖指導教師：趙宗勝20080601AbstractChicken—quailcrossiSaclassichybridizationinfowlbutthehybridizationexistedincompatibilitytOsomeextent，mountsofembryosdi

2、edattheearlystagesofincubateFromourexperiments，wemayconcludethatthegenescorrelatedsexdifferentiationfromchickenandquailaredifferentexpressive，duringthesexdifferentionstages，leadtothedeathofhybridsembryosAnd，wesupposedtha

3、tERanditsrelategenesmayplayallimportantroleinthedeathsOnaccountofabove，wedoourexperimentsfromthreeaspects：1ObservationofPGCsinearlyembryosofgenetichybridsofchicken—quailToobservePGCsinearlyembryosofgenetichybridsofchicke

4、n—quail，weadoptthemethodofhistoiogicalsectionandPASSpecificstainingTheresultsshowedthatPGCsofchickembryosiSeasytobeidentifiedthroughPASSpecificstaining，butitiSdi療iculttOidentifythePGCsintheembryosofgenetichybridsofquaila

5、ndchickenquailMavbePGCsinearlyembryosofhybridsjSthesarneasquails’PGCs，becausePGCsinquailhaslittleglycogen，SOitiSdi幣culttobestainnedwithPAS2TheeffectanddifferentexpressionofAromataseGeneinearlyembryosofgenetichybridsofchi

6、ckenquailTheenzymeAromatase，thatcatabolizesthetransformationofandrogensintoestrogens，isarestrictenzymeintheprocessWehadcollectedearlylivingembryosof84、96、102、108、114、120、132hatrandomAdoptedthemethodofRTPCRtaken13actinasi

7、ntemalstandardtOdeterminedtherelativequantityofAromatasemRNAonthefoundationofembryossex0urresearchindicatethattranscriptsweredetectedintheembryosoffemalebutnotmaleembryosfrom84hAt132hgettotheMaximumAtthetimeofl32h，therei

8、snofemaleembryosnearlyAromataseisthefemalespecialexpressgene3SitesofERExpressioninearlyembryosofgenetichybridsofchickenquailPreviousreseachjnearlyembryosofhybridsofchickenquailindicatethatthegonaddi仃erentiationofhybridsw

9、as6684hofembryosdevelopmentERexpressedabnormallyandthefemaleERmRNAexpressionweresignificantlyhigherthanmaleintheperiodsofgonaddifferentiation，withthemethod0fRrPeRWithⅥmolemountlSH56、66、72、84、96hearlylivingembryosbecollec

10、tedatrandomfortheexperimentWefoundthatERWaSexpressedaroundthegenitaIridgeatthetimeof6684hbutdidn’tfoundlargelyexpressinthetimeof56and96hKeywords：chicken，quail，earlyembryosofhybrids，PGCs，Aromatase，ERTypeofpaper：A(theoryst