C666-1和CNE2細(xì)胞的HMME-PDT殺傷效應(yīng)實(shí)驗(yàn)研究.pdf

1、福建師范大學(xué)碩士學(xué)位論文C6661和CNE2細(xì)胞的HMMEPDT殺傷效應(yīng)實(shí)驗(yàn)研究姓名：陳爭申請學(xué)位級別：碩士專業(yè)：光學(xué)指導(dǎo)教師：李步洪20090401摘要AbstractNasopharyngealcarcinoma0we)hasastrikinggeographicalandethnicaldistribution，anditOCCllrS謝廿1highfrequencyinsouthernChinaandSoutheastAsiaT

2、hepre—clinicalandclinicalstudiesbasicresearchandclinicalapplicationofphotodynarnictherapy(PDDonnasopharyngealcarcinoma(NPC)NPCwerepresentedindetailedTodate，ingreaterthan95％ofpatients謝廿lNPC，thelatentformofEBVispresentiIlt

3、heNPCcellsHoweverthepreviouscelllineshatusedforPDTstudies，suchasCNE1，CNE2andHKlwereNPCcelllines也athaveshedtheirEBVInthiscase，theC666一lNPCcelllinehasconsistentlyandwidelybeendemonstratedtoharborthelatentformofEBVaroundthe

4、worldInordertocomparethePDTefficiencyofhematoporphyrinmonomethylether(HM施)mediatedPDTforNPCC6661andCNE2celllines，laserinducedfluorescencetechniqueandconfocalmicroscopewereusedtostudytheaccumulatedconcentrationandsubcellu

5、larlocalizationofHMMEinC666—1andCNE2cells，respectivelyThesurvivalrateofC6661andCNE2ceilsafterHMMEmediatedPDTWasevaluatedwithcolonyformingassay111eresultsindicatedthatthecellularHMMEconcentrationincreased謝也theincubationti

6、meinC6661andCNE2ceils，andthesaturationhappenedaround5hAscomparedtotheC666—1cells，CNE2cellshadahigheraccumulatedconcentrationafterthesameincubationtimeThereWaSnosignificantdifferenceforthesubcellularlocalizationofHMMEinbo

7、thC666一landCNE2cells，andHMMEhasadiffusecytoplasmicdistributionThehigherconcentrationofHMMEinCNE2cellsinducesthehighersurvivalratefollowingPDTInconclusionthePDTefficiencyofH^n嗄EmediatedPDTforCNE2cellswashigherthanthatforC