2023年全國碩士研究生考試考研英語一試題真題(含答案詳解+作文范文)_第1頁
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1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文非同步移植胚胎的著床及SNAIL和COX2在著床位點的表達(dá)姓名:李世杰申請學(xué)位級別:碩士專業(yè):基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師:楊增明20040501東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文THEIMPLANTATIoNoFASYNCHRONoUSLyTRANSFERREDEMBRYoSA^『DTHEEXPRESSl0N0FSNAILANDCoX2ATTHESlTEoFIMPLANTALTl0NABSTRACTBlastocystimpl

2、antationisakeystepforSUCCESSfulestablishmentofpregnancyduringmammalianreproductionSuccessfulimplantationisdependentupontheinteractionbetweentheblastocystandtheuterusThemostfundamentalfeatureofthisprocessiSthesynchronized

3、developmentoftheembryototheblastocyststageanddifferentiationoftheuterustothereceptivestateTheexpressionofimplantationrelatedmoleculeshasnotbeenexaminedafterasynchronousembryotransferInthisstudy,fourgroupshadbeendesigned,

4、including(A)Zygotesand8cellstageembryosweretransferredseparatelyintotheoviductsofthesamerecipientOnday1ofpseudopregnancy;(B)Embryosofzygotesandblastocystsweretransferredseparatelyintotheoviductsofthesamerecipientonday1of

5、pseudopregnancy;(C)Morulaweretransferredintotheoviductsoftherecipientonday1ofpseudopregnancy;(D)Blastocystsweretransferredintotheoviductsoftherecipientonday1ofpseudopregnancyEmbryoimplantationwasexaminedatI6:00and20:00of

6、day4,andat8:00ofday5byintravenousinjectionofChicagobluedyeTheexpressionofbothSNAILandCOX2atimplantationsiteswasexaminedbyimmunohistochemistryInthisstudy,theembryosasynchronouslytransferredcouldimplantinalloffourgroups,On

7、day5posttransferthesizeofimplantedembryosbetweeneachexpedmentalgroupsWasdifferent,thedifferenceingroupAandbetweenzygoteandmorulatransferredinsamedayWasverysignificant(PO01),thegroupBthatthesizeofimplantedzygoteswassignif

8、icantlysmallerthanthatofblastocysts(P005)Themorphologiesofimplantedembryosweredifferentbetweeneachgroup,havingtheembryosfromattachedblastocyststoeggcylinder,TheimmunostainingofbothSNAILandCOX2wasalsodifferentineachgroupW

9、hentheembryosweretransferredat8cell,morulaorblastocyststage,theimmanostainingforSNAILandCOX2wasstrongerthanthattransferredatzygotestageTheseresultssuggestthattheembryostransferredatadvancedstagecouldimplantpriortoyounger

10、embryosandimplantation—relatedmoleculeswerealsoexpressedatimplantationsitesofmouseuteruswhentheadvancedembryoswereimplantedPostgraduate:LiShijieSpecialty:DeveIopmentalBiologySupervisor:ProfessorYangZengMingKeywords:mouse

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