惡性腫瘤轉(zhuǎn)移血管生成依賴性學(xué)說的探索性研究.pdf

1、四川大學(xué)博士學(xué)位論文惡性腫瘤轉(zhuǎn)移血管生成依賴性學(xué)說的探索性研究姓名：黃欣申請學(xué)位級別：博士專業(yè)：口腔臨床醫(yī)學(xué)指導(dǎo)教師：溫玉明20030420型叢塑塑鱉鱉型：MetastasisofMalignantTumorIsDependentOnTheAngiogenesisTutorMDcandidateProfessorWenYu—MingHuangXinEnglishAbstractIAbstract】Objective：Metastasis

2、isthemostimportantinthestudyofmalignanttumorsAsbeenshowed，angiogenesisisveryimportantinthecoursesofproliferation，invasionandmetastasisofmalignanttumorsVEGFisresponsibleforangiogenesisTllisstudyistofindtherelationshipbetw

3、eenVEGFandmetastasis，thuswecarlknowwhetherthemetastasisofmalignanttumorisdependentontheangiogenesis，andtosupplysomeusefulinformationsfordoctorsintherapyingtumorsMethods：l，DetecttheVEGFlevelssecretedbydifferenttumorcellli

4、neswithdifferentmetastasispontency，withtheELISAmethod；2，DetecttheLNandIVtypecollagenlevelssecretedbyendothelialcellsafterputtingVEGFintothecultures，withtheradioimmunitymethod；3，Inoculatetumorcellsintonudemices，andtheninj

5、ectVEGFtonudemices，calculatethemetastasisratio；4，DetecttheVEGFlevelsinthepefipheralbloodandReMVDinthe1ymphnodesofpatientswithtonguecancerbythemethodofELISAandimmunohistochemicaltechniqueResults：1，VEGFlevelsaredifierentin