來源于人樹突狀細胞和骨髓基質(zhì)細胞的兩個新分子CRL2和CLM的克隆與功能的研究.pdf

1、浙江大學博士學位論文來源于人樹突狀細胞和骨髓基質(zhì)細胞的兩個新分子CRL2和CLM的克隆與功能的研究姓名：孫紅穎申請學位級別：博士專業(yè)：腫瘤學指導教師：曹雪濤20040601塑i墮堂堡主蘭垡壘塞Identificationoftwonovelmolecules，typeIcytokinereceptorCRL2fromhumandendriticcellsandCystatin—likemolecule(CLM)frombonemarro

2、wstromalcellsAbstractPanI、ExpressionpatternandfunctionalstudyofanoveltypeIcytokinereceptorCRL2clonedfromhumandendriticcellsDelldrificcellsarethemostpotentantigen—presentingcellsinhumanimmunesystemBylargescaredrandomseque

3、ncing，weisolatedalotofnovelfull—lengthcDNAsfromtheeDNAlibraryofhumandendriticcellsInaddition，wealsoconstructedaeDNAlibraryofbonemarrowstromalcells(BMSC)andthenisolatedseveralnovelfull—lengthcDNAsInthepresentstudyweidenti

4、fiedorinvestigatedthebiologicalfunctionsoftwonovelmolecules，onefromDCandanotheronefromBMSCNoveltypeIcytokinereceptorCRL2wasfirstlyclonedfromDCcDNAlibrarybyus(BBRC，2001)SeveralmonthslaterscientistsfromDNAXresearchinstitut

5、edemonstratedthatTSLP(Thy’micStromalLymphopoietin)isacytokineanditsreceptorclonedashTSLPRBydoinghomologysearchandcomparinghTSLPRwithCRL2，wefoandhTSLPRistheCRL2ThescientistsfromDNAXresearchinstitutedidaIotofworkonTSLPandf

6、oundalotofinterestingphenomenon：humanTLSPfunctionsmainlyonmyeloidcells，humanTLSPenhancesthematurationofCD11cDCandmaintainstheirsurvivalTSLPactivatedDCmediatebothCD4andCD8TcellresponseswithaproallergicphenotypeMiceoverexp

7、ressingTSLPdevelopmixedcryoglobulinemiawithrenaldiseasecloselyresemblinghumancryoglobulinassociatedmembranoproliferativeglomemlonephrit!s(MPGN)，includingglomerulardepositsofimmunoglobulinsandcomplementAllofaboveresultsin