年齡相關(guān)性聽力減退大鼠聽皮層、蝸核、耳蝸中SOD、MDA的變化及神經(jīng)細(xì)胞凋亡初步觀察.pdf

1、中南大學(xué)碩士學(xué)位論文年齡相關(guān)性聽力減退大鼠聽皮層、蝸核、耳蝸中SOD、MDA的變化及神經(jīng)細(xì)胞凋亡初步觀察姓名：周媛申請學(xué)位級別：碩士專業(yè)：耳鼻咽喉科學(xué)指導(dǎo)教師：謝鼎華20060501碩士學(xué)位論文英文摘要AbstractsObjective：Toexplorethechangesofsuperoxidedismutase(SOD)malondialdehyde(MDA)andapoptosisintheauditorycortex，the

2、cochlearnucleiandthecochleaoftheratswithagerelatedhearingloss，andunderstandtherelationshipbetweenoxygenfreeradical(OFR)，lipidperoxiclationandapoptosiswithpresbyacusisMethods：TIlreemonth—oldrats(ABR205dBSPL、andeighteenmon

3、th—oldrats(ABR5510dBSPL1wereusedinthestudyT_SODactivitywasmeasuredbyxanthineoxidasep(0)methodMDAlevelwasexaminedbvthibabituricacid(TaA)methodApoptosisWasexaminedbyAnnexinVFITC／PIstainingwithflowcytometry(FCM)Results：1neS

4、ODactivities(U／mgprot)intheauditorycortexthecochlearnucleiandthecochleaoftheeighteenmontholdratswere170331615。366357910，382568810，andthelevelsofMDA(nmol／mgprot)were179017，316024，166024respectivelyHowevertheSODactivities(

5、U／mgprot)intheauditorycortex，thecochlearnucleiandthecochleaofthethreemontholdratswere267322010，483976025，479439025，andthelevelsofMDA(nmol／mgprot)were126O10，242031，131033respectivelyTheSODactivitiesdecreasedandtheMDAlevel

6、sincreasedsignificantlyintheauditorycortex，thecochlearnucleiandthecochleaoftheeighteenmontholdrats佃O005、2Theapoptosisrates(％)intheauditorycortexandthecochlearnucleioftheeighteen—montholdratswere6238999，27812431。andtheapo

7、ptosisrates(％)intheauditorycortexandthecochlearnucleiofthethreemontholdratswere4070872、627192respectivelyTheapoptosisratesincreasedsignificantlyintheauditorycortexandthecochlearnucleioftheeighteen—montholdratsrP005)Concl

8、usions：TheresultsshowedthattheSODactivitiesintheauditorycortex，thecochlearnucleiandthecochleaoftheeighteenmontholdratsdecreasedsignificantlyandtheMDAlevelsinthesametissuesincreasedsignificantlyTheapoptosisratesincreasedⅡ