Ad-hNIS體外轉染肝癌細胞介導放射性碘攝取的研究.pdf

1、華中科技大學碩士學位論文AdhNIS體外轉染肝癌細胞介導放射性碘攝取的研究姓名：韓云峰申請學位級別：碩士專業(yè)：影像醫(yī)學與核醫(yī)學（核醫(yī)學）指導教師：趙明20090501華中科技大學碩士學位論文華中科技大學碩士學位論文表達的hNIS蛋白功能。方法：方法：體外復蘇、培養(yǎng)人肝癌細胞系MHCC97，接種于培養(yǎng)瓶。用AdNIS轉染人肝癌細胞MHCC97做為實驗組，同時設空病毒轉染的陰性對照組和未加任何病毒的空白對照組，動態(tài)檢測131I的攝取、觀測K

2、ClO4對碘攝取抑制情況。結果：結果：轉染AdNIS的肝癌細胞MHCC97能迅速攝取131I，其攝碘功能可被KClO4特異性抑制。結論：結論：hNIS基因通過轉染重組腺病毒的方式導入人肝癌細胞系MHCC97表達了功能性hNIS蛋白，為下一步的體外和體內治療實驗奠定了實驗基礎。關鍵詞：關鍵詞：人鈉碘同向轉運體重組腺病毒轉染人肝癌細胞放射性碘ThefirstpartAdhNIStransfectionofhumanhepatomacells

3、invitrothedetectionofthehNISgeneexpressionlevelinthecellsAbstractObjectiveTodetecthumansodiumiodidesympter(hNIS)expressioninHumanhepatocellularcarcinomacelllinesMHCC97Methods1、Humanembryonickidneycelllines(HEK)293cellswe

4、rethawedculturedinoculatedinculturebottlesinvitro.AdhNISwasamplifiedtotherequiredvirustiterbyVirussupernatantwhichwasfromAdhNISlesioncellsinfectingHEK293cellsinlogarithmicphaseoveroveragain.thentheinfectiontiterofrecombi

5、nantadenoviruswasevaluated.2、MHCC97cellswerethawedculturedinoculatedin12wellplatesinvitro.Differentwellwasgivendifferentvirusmultiplicityofinfectionthecells’responsewereobservedundermicroscopein2448hourtodetermineoptimal

6、multiplicityofinfection.3、MHCC97cellswerethawedculturedinoculatedinculturebottlesinvitro.ThecellsweredividedintoanexperimentalgrouptransfectedwithAdhNISacontrolgrouptransfectedonlywithemptyvirusacontrolgroupwithnovirusin