乳酸菌胞外多糖的提取純化及免疫活性研究.pdf

1、山東大學(xué)碩士學(xué)位論文乳酸菌胞外多糖的提取純化及免疫活性研究姓名：王靜穎申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：汪天虹20070518AbstractExopolysaccharide(EPS)，onekindofcarbohydrates，issecretedbylacticacidbacteriaduringitsgrowthmetabolismAsulnnlalyofexopolysaccharidewasshowedinthis

2、paperreflectinginitschemicalcharacteristics，physiologicalfunctionapplicationandthefactors，whichaffecteditsyield鶘wellBesidestheresearchesonEPSproductionfromStreptococeasthermophihosandLactobacillusbulagaricus，capableofsec

3、retingexopolysaccharides，aleconductedinproductionconditionextraction，purificationanditsimmunologicalactivitiesneproductionconditionsofEPSwereselectedduringtheprocessofproducingyoghurtItshowedthatthereisthehighestyieldofE

4、PSwhenseedvolumeis2％，fermentationtemperaturereachedto38“C，fermentationlastedfor25handloweraciditypresentedattheendoffermentationInthemeantime，theeffectsofimprovementofculttaemediumbyaddingthejuiceofapple，tomato，strawberr

5、yandgrapeontheyieldofEPSalestudiedItindicatedthatallthefourjuicesalenotonlythegrowthpromotingsubstancesforlacticacidbacteriabutalsotheyield—raisingfactorsforEPSTherewasadeproteinizationprocedureduringtheprocessofEPSfromy

6、oghurtThedeproteinizationusingSevagmethodtwiceafterenzymolysisresultedinthepurifiedEPSThisprocedurecouldincreasetheextractionrateofEPSanddecreasedtheuseoforganicsolventEPSwasextractedfromtheculturesofStreptococeasthermop

7、hihosandLactobacillusbulagaricusgrowninMRSmediumThepurificationofEPSWascarriedoutnleimmunologicalactivityofEPSwasstudiedbyusingmouseinbredlinesasexperimentalmaterialThissubstancefromyoghurtdidnotaffecttheweightofmouseimm