牛流行性白血病分子病理及其應(yīng)用研究.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文牛流行性白血病分子病理及其應(yīng)用研究姓名：龍塔申請(qǐng)學(xué)位級(jí)別：博士專(zhuān)業(yè)：預(yù)防獸醫(yī)指導(dǎo)教師：佘銳萍潘耀謙20041201AbstractEnzooticbovineleukemia(EBL)isaninfectiousneoplasmcausedbybovineleukemiavirus(BLV)ThisdiseaseisfoundinallcountriesofworldwhichraisecowsThereisas

2、ernmpositiveratefrom30％to50％inSOl／lecattlegroupsinourcountryItcausesthreateningforraisingcattleinChinaUptonowthereisnomethodtocontrolandtreatthisdiseaseTheaimofstudyonappearanceofEBLismainlycentralizedonBLVTheknowledgeof

3、genesexpressionisafewinthefunctionofEBLandaspectsindiagnosisandtreatmentThisstudythatobservedtheexpressionanddistributionof4apoptoticfactors，relationapoptoticfactorwithapoptosis，changesofTandBsubgroup，andchangesofmolecul

4、arpathologyin14cowssufferingfromenzooticbovineleucosisfromseveralresideswasdetectedbyhistochemistryTUNELtechnologyimmunefluorescentflowcytometryandsoonTheaimofthisexperimentsuppliestheoreticbasisforpreventiveandtherapyof

5、EBL，andconsuRdatesforTlymphoidcellleucosisofhumanbeingTheresuRsweredescribedinthefollowing，Theneoplasmlesionswerefoundinalllyrephnodesofbody(especiallyinnecksuperficiallyrephnode，mesenterylymphnodeanddiaphragmlymphnode)a

6、ndalotoforgans(cgheart，stomachandkidneysandsoon)MicroscopicallythepervasionoftumorcellstotheorgansandtissueswasfoundthroughthelymphvesselsandbloodvesselsThesuggestionswereputforwardthatitshouldgettostrengtheninspectionin

7、EBL，treatasusinginindustryandnoeatingforslaughteredbedywithtumorsinalllymphnodesandorgansThepositivecellsexpressionofP53hadanimportantrelationtotheappearanceofEBLBcellswithP53mutationstrainmightbeatargetcelltoformattumor

8、sBaxandCaspase3hadahighexpressionintumorcellswithmoredifferentiationandapositiverelationtotumorcellapoptosisfP001)ThepositiveexpressionintensityofBcl2hadarelationtodegreeoftulnorcelldepravationandhadanegativerelationtotu

9、morcellapeptosisrPO01)ThisfindingmightofferanewwaytostudymolecularmechanismofdevelopmentintumorcellsandanewtheoryfordiagnosisandgenestreatmentofEBLinclinicThisstudyindicatedthat14caseshadacharacteristicoflinesBandruinere

10、elloriginfromBlacellsCDl9，CD20，CD45RAandCD45RBwerethereliablesuperficialsignsTheabnormalityofTcellswasfoundinearlewithEBLOwingtolossequilibriumofCD4cellandCD8cellandreducecounteractiontotmnorcells，theruinerappearedinbody

11、Therefore，reinforcingbreedingandheighteningresistibilityhaveimportantmeansinpreventingEBLThisstudyshowedthatthetumorcellsmighthaveabilitytosecreteTNFa，VEGFandCTTheyhadahigherexpressiveratethantumorsidewardtissuesandhadas

12、ignificantlystatisticdifferencebetweenbothtumortissuesandtumorsidewardtissuesrP001)AmongthempositiveexpressionratioofBEGFwas786％andTNF—awas429％InvirtueofVEBFmoreexpressionnewvesselsintumortissuescouldgrowandledtotumorcel

13、linfiltrationtoalotoftissuesTheresultsindicatedthatlevelofVEGFandCTintumortissuesmightmakeasasigntodiagnosisofEBLVEGFmightalsomakeavaluablejndexofrecrudesceandtreatmentoftumorinclinicTheformationofantivesselshadaimportan