豬流行性腹瀉病毒主要結構蛋白不同抗原片段在重組干酪乳桿菌中的表達及其免疫學評價.pdf

1、ClassifiedIndex:U.D.C:DissertationfortheDoetoralDegreeinAgrieultureEXPRESSIONOFTHEDIFFERENTANTIGENFRAGMENTSOFTHEMAJORSTRUCTURALPROTEINOFPORCINEEPIDEMICDIARRHEAVIRUSINRECOMBINANTLAC刃OBACILLUSCASEIANDIMMUNOLOGYEVALUAr1ONCa

2、ndidate:GeJunweiSupervis:prof.Li兩ingAeademicDegreeAPPliedfor:DoctorofAgrieultureSPeeiality:PreventiveVeterinaryMedieineDateoforalExamination:June.2008Universi好廠:NortheastAgriculturalUniversity東北農業(yè)大學農學博士學位論文旦旦旦旦旦旦旦旦旦旦旦旦旦旦

3、旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦魚魚魚魚魚魚魚魚魚旦魚魚魚少夕頤坦貝坦旦魚旦旦..口，..夕口，甲.口甲夕甲口坦旦魚魚魚魚魚旦!旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦旦3.實驗結果.............................................................................................................

4、……343.1又FPCR結果.................................................................................................................……343.2測序結果與序列分析.............................................................................

5、......................……343.35基因克隆結果............................................................................................................……，383.4大腸桿菌中外源蛋白表達及純化......................................................

6、.........................……383.5抗血清滴度檢測.............................................……，......................……，..........................……393.6中和試驗.......................................................................

7、................................................……403.7重組表達載體的鑒定...................................................................................................……403.8重組乳桿菌的表達鑒定............……，.........................

8、...................................................……433.8.1細胞表面表達型重組干酪乳桿菌的誘導表達鑒定結果.......................................……433.8.2分泌表達型重組干酪乳桿菌的誘導表達鑒定結果...............................................……503.9免疫小鼠后特異性抗體檢測結果

9、.......................................……，...................................……553.9.1小鼠血清中特異性IgG檢測結果..........................................................................……553.9.2免疫小鼠鼻沖洗液中特異性slgA檢測結果...............…

10、…，...................................……593.9.3免疫小鼠糞便中特異性slgA檢測結果.................................................................……633.9.4免疫小鼠外生殖道沖洗液中特異性slgA檢測結果..........……，……，.....................……673.9.5免疫小鼠眼沖洗液中特異性s

11、lgA檢測結果....................................................……，.…713.9.6腸粘液中特異性slgA檢測結果............................................................................……753.10特異性淋巴細胞增殖試驗.......................................

12、....................................................……773.11細胞因子測定結果.....................................................................……，...........................……793.12口服重組干酪乳桿菌免疫小鼠所獲得的血清抗體及腸薪液抗體中和試驗結果....……794.討論

13、......................................................................................................................……824.1PEDVLJB03株s和n基因的分子特征……，............................................................……824.2課題設計思

14、路...............................................................................................................……824.3PEDV抗原片段篩選及其意義..............……，...............................................................……854.4

15、表達不同抗原片段的重組乳酸桿菌的免疫效果分析.....……，，…，..............................……864.5提高乳酸桿菌轉化效率的關鍵技術...........................................................................……905.結論....................................................

16、................................……，.........................……93致謝..................................................................................................................……94參考文獻................................

17、..............................................……，.............................……95附錄................................................................................................................……104攻讀博士學位期間發(fā)表的論文......