運輸和抗應(yīng)激添加劑對肉仔雞生理生化指標(biāo)和肝臟HSP70基因表達及肉質(zhì)的影響.pdf

1、甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文運輸和抗應(yīng)激添加劑對肉仔雞生理生化指標(biāo)和肝臟HSP70基因表達及肉質(zhì)的影響姓名：付曉申請學(xué)位級別：碩士專業(yè)：動物營養(yǎng)與飼料科學(xué)指導(dǎo)教師：汝應(yīng)俊劉國華20080601甘肅農(nóng)業(yè)大學(xué)2008屆碩士學(xué)位論文SummaryTwoexperimentswereconductedtoassesstheeffectofstockingdensityofbroilersduringsimulationtransportationd

2、urationandantistressfeedadditiveonplaysiologicalandbiochemicalparameters，heatstressproteinHSP70expressionandmeatqualityInexperiment1，totalof48malebroilersat49dweredividedinto5groups，晰tll8證treatment13，5(10wstockingdensity

3、8malebroilers／064mz)，and12intreatment24(tlighstockingdensity12malebroilers／O64m2)ThetransportationWasappliedbyusingsimulatingtransportationmodeTreatmentland2werestandingfor2h，treatments2and4weretransportedfor2h，treatment

4、s3and5weretransportedfor4hTheresultsshowedthattransportationstressdecreasedtheleveloftheserurflT3(PO05)，butdidnotinfluenceCORWiththetransportationtimebeingprolongedandstockingdensitybeingincreased，serumINSgraduallydecrea

5、sednumericallywiththehi曲density／transport4hbeingthemostaffectedgroupMeanwhiletransportationincreasedthemuscleglycogencontent(PO05)TransportationstressresultedinanincreaseofbreastmuscleandlegmuscleMDAMDAWasincreasedwithhi

6、ghstockingdensityanddecreasedwiththeprolongedtrasportationtimeTransporationstressalsoincreasedtheactivityofmuscleNOSandSOD，andtheactivityofSODWasincreased、析mtheincreaseintranportationtimeandstockingdensityForthetreatment

7、of4htransportationhighstockingdensityresultedinahi曲SODcontentinbreastmusclethanthelowstockingdensity11letreatmentof4htransportationatahighstockingdensityhadahigherSODcontentinlegmusclethanthe2htransportationatalowstockin

8、gdensityTransportationfor2himprovedHSP70mRNAAbundance(P005)，buttransportationfor4hHSP70mRNAAbundancereducedbacktothenormallevelTransportationstressreduceddriplossofbreastmuscleandlegmuscleat24h，breastmusclepHandlactatele

9、vel，increasedthedriplossofbreastmuscleandlegmuscleatlh，pHandlevelinthelegmuscleThisresultindicatedwhiletransportationstressCallinducetissueperoxidationinjurybroilershadthecapacityofadaptingtothetransportationstressAndHSP