抗真菌抗生素生產(chǎn)過程中利迪鏈霉菌蛋白質(zhì)組研究.pdf

1、天津大學(xué)碩士學(xué)位論文抗真菌抗生素生產(chǎn)過程中利迪鏈霉菌蛋白質(zhì)組研究姓名：王偉申請學(xué)位級別：碩士專業(yè)：生物化工指導(dǎo)教師：元英進(jìn)20080101ABSTRACTTowellunderstandthegro、vthandproductionof&r印幻，礁yc酷紗沈c螂AS42501f而mshakeflaskto30LfernlentorduringantifungalactiVitiesproduction，、winvestigatedthe

2、capabili夠ofantifungalactiVities，changesinintracellularandeXtracellularpmteome，and10wmolecularmetabolitesResultsareasfollowing：Theantifungalbioactivit)，ofeXtracellularcompondsandintercellularcompondsofS如疣c凇AS42501andS咖擊c淞

3、E9wereexaminedWhetherextracellularcompoundsorintracellularcompounds，thebioactivityof最咖歷伽E9ismuchhigherthanthatofAS425012DEanalysisshowedthatthereissigni6cantdifferenceinintraandextracellularproteinsbetweenS。紗歷c淞AS42501an

4、dS6砌c螂E9The22signiflcantlyexpressedproteinswereidentifiedusingMALDl—TOF—MSTheseproteinsareassociatedwithbioprocessesofgene們nscription，carbohydratemetabolism，phosphoIipidmetabolism，andbiosynthesisofantifungalantbiotic，suc

5、hastetRfamiIytranscriptionalregulator，ATPdependentCIpproteaseproteolyticsubunit，phosphogIyceromutase，phospholipase，deoxyuridine5’triphosphatenucleotidohydrolase，polyphosphateglucokinase，glucose6一phosphate1一dehydrogenase，

6、andsoonIntercellularandextracellularproteomeof＆紗西c“sE9in2Land30Lfemlentorwerefurtherinvestigated2一DEpronlesweredifferentindifferentfermentorsatdifferentculturestagesThe23proteinsincludingcarboxylase，cetylCoAacetyltransfe

7、rase，acetyltransferase，omithinecarbamoyltransferase，adeninephosphoribosyltransferase，andglutaminesynthetasewereidenti6edTheidentmedproteinswererelatedtothep01yketidepathwayTherefore，wepresumethatthepolyketidepathwaywaspo

8、ssiblethemainbiosyntheticpathwayofthisantifungalantibioticThelowmolecularmetabolitesofS紗講c“swereprimarilyexploredusingGCTOFMSPCAanalysisreveal5me協(xié)bolitescorrespOndingtoglycerolbutanetenol，DGalactose，glucitol，DTuranosePos