30608.基于16srdna水平的海洋厭氧活性菌株的分類學(xué)研究

1、大連交通大學(xué)碩士學(xué)位論文基于16SrDNA水平的海洋厭氧活性菌株的分類學(xué)研究姓名：付步飛申請學(xué)位級別：碩士專業(yè)：環(huán)境科學(xué)指導(dǎo)教師：穆軍20090605人連交通人’學(xué)I：學(xué)碩f：學(xué)何論文fillimlAbstractTaxonomicresearchaboutanaerobicactivityofstrainsofmarine，notonlyhelpsUStoexplorethemarineenvironmentoftheresource

2、s，expandsthetypesofnewspeciesandquantity，butalsoisthenecessaryconditionaboutthestudyofmarineanaerobeantibioticsThereiSvarietyofmarinemicroorganismsintheoceanBecauseofthediversityofthelivingenvironment，suchaslight，oxygen，

3、pressureandotherfactors，marinemicroorganismshavethedifferentmorphologicalstructureandphysiologicalfunctionsAndabout80％areanaerobicones，suchasphotosyntheticbacteria，sulfatereducingbacteria，denitrifyingbacteria，methane—pro

4、ducingbacteria，etcTheenzymaticsystemofmetabolismisbigdifferentbetweenmarineaerobicmicroorganismsandanaerobiconesIntheory，thesecondarymetaboliteswillbeagreaterdifferencewhichproducedbydifferentmetabolismanddifferentrespir

5、atoryprocessesThereforethetaxonomyresearchofthemarineanaerobicmicroorganismshasthevitalsignificancetothenewantibiotic’Sscreeningandthenewmedicine’SdevelopmentInthispaper，29strainsofmarineanaerobicbacteriawereisolatedfrom

6、samples，including9strainsofmarinephotosyntheticbacteria，16strainsofmarinedenitrifyingbacteria，4strainsofthemarinemanganese—oxidizingbacteria5ofthemhadbeenidentifiedby16SrDNAResultsshowthatthefivestrainshavesignificantdif

7、ferencesinmorphologicalcharactersThemarinebacteriumDN7wasidentifiedasPseudomonasstutzerfwhichhastheobviousinhibitiontoBacillussubtilisandPseudomonasaeruginosaThereisonly97％similaritybetweenmarinebacteriumDN3—1’S16SrDNAan