農(nóng)桿菌介導(dǎo)三個(gè)Bt基因在YZ1棉中的遺傳轉(zhuǎn)化.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文農(nóng)桿菌介導(dǎo)三個(gè)Bt基因在YZ1棉中的遺傳轉(zhuǎn)化姓名：覃春申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：作物遺傳育種指導(dǎo)教師：郭小平201106華中農(nóng)、Ik_人學(xué)201I屆碩上研究生學(xué)位論文AbstractUplandcotton(GossypiumhirsutumL)isoneofmostimportanteconomiccropsinChina，butalsoonecroptendstoinfestedbymanykindsofpes

2、ts，withthedamageof15％20％everyyearInsectpesthasbecomeoneofthethemainfactorsofinfluenceofcottonproductionOverthepastdecadespesticidesarewidelyusedinthepreventionandcontrolofthepestswhichhavecausedseriesproblemssuchasenviro

3、nmentalpollution，ecologicalunbalanceTranditonallytheApplicationofinsectresistantcultivarbasedonmorphologicalandbiochemicalpropertiesishelpfultogetgoodeconomicandsocialbenefitsbyreducingtheinvestmentofpesticides，Withthede

4、velopmentofeconomicandsocietymodemagriculturerequkeCottonvarietiesmorequalitysuchasHighyield，highqualityoffiber，diseaseresistance，insectresistance，droughtresistanceandSOonBecauseofnarrowgeneticbasis，itisdifficulttoimprov

5、etheinsectresistancefurtherlythroughtraditionalhybridbreedingmethodsinChinaThedevelopmentofbiologicaltechnologyprovideanimprotantwayforinsect—resistantbreedingIngeneticengineering，afewresistantgeneshavebeenwindlyusedtoge

6、neticallyimprovecoRonresistancetoinsectwhichincludBacllusthuringensis(BO，ProteaseInhibitors(pI)genes，Amylaseinhibitorsgenes(AI)，LectingeneetcInthisstudythreeBtgene(CryIC，CrylIA，Cry9C)weretransformedintoYZlltoimprovethele

7、velofinsectresistancethroughagrobacterium—mediatedmathodThehypocotylofYZIandC312werechosesedtobeinfectedbyEHAl05whichcarrythethreeBtgenesinthisresearch，andthencultivatedonscreeningculturemediumcontainingO1mg／L2，4一D01megL

8、KTExperimentsshowthatcallusCanbeavailablequicklyhoweverregenerabilityofembryonalcallusvarieddifferentlybetweenYZIandC312indifferentialmediumfourmonthslaterCallusofYZIdifferentiatedintoembryogeniccallus，andthenacompletedp

9、lantregenerationyethard，green，compactcallusWasproducedonlybyC312atthesanletimeInthefieldweinitiallyidentifiedfourtransgeniccottonsbyO8％bastaselectionPCRanalysisfurtherconfnmedthatbothCrylIAandCry9Chadbeenintegratedintoco

10、ttongenomeTheresultofinsecttestshowedthatthebothofBtgeneshavehighresistancetoHelicoverPaarmigerawhosegrowthanddevelopmentweresignificantlysuppressedwitninfourdaysThetestalsopointedoutthatdifferenttransgeniccottonsperform