嗜熱鏈球菌超濃縮培養(yǎng)及生物學變化規(guī)律.pdf

1、東北農(nóng)業(yè)大學碩士學位論文嗜熱鏈球菌超濃縮培養(yǎng)及生物學變化規(guī)律姓名：袁肖寒申請學位級別：碩士專業(yè)：基礎獸醫(yī)學指導教師：高學軍20050501東北農(nóng)業(yè)大學農(nóng)學碩1嶼。位論文Super—concentratedIncubationandBiologicalChangesofStreptoccusThermophilusAbstractInordertodevelopasuperconcentratedincubationmethodofStr

2、eptococcusthermophilus晦0，theresearchwasconductedtoscreenthemainnutritivesubstancesfirstlyonthebasisofbasicmedium，andthentoscreenthemultiplicationfactors(MFs)，asaresulttheingredientsofsuperconcentratedsubstratumweredeterm

3、inedThemetabolicregularitiesofStreptococcusthermophilusonthenutritivesubstancesofsaccharine，proteinandfatintheculturemediumandthe北angesofultrastructureandtheapoptosisduringsuperconcentratedincubationweresrudi列byapplyingt

4、hemethodofcentrifugesemi—continuousprocessandbythesupermicroscope，TUNELstainingandagarosegelelectrophoresis(AGE)，respectively，andtheresearchprovidedthefoundationforthesuperconcentratedincubationtechniqueofStreptococcusth

5、ermophitusInthepaper，theenrichmentcultureformulaofStreptococcza“therTnophitusanditsculturalconditionweredeterminedTheculturemediumcontainedthefollowingingredients：15％lactose025％glucose，1，5％soypeptone，1O％lactoalbuminhydro

6、lysate，15％yeastextractpowder35％salinesolution，015％vitaminc，01％／yslne，005％glutamate，O05％arginine，5％maltwortand5％tomatoextractTheenrichmentprocedurewasfollowedbysemicontinuousapproachandthebacteriacountcouldreachupto103210

7、10cfu／mLbytheculturemethodofsecondarycentrifugation，about10timeshigherthanthebasalculturemethod，anditwasprovedtobeaveryeffectivesuperconcentratedincubationmethodforStreptococcusthermophilusInconclusion，thelagphaseofStrep

8、tococcusthermophiluswasshortenintheeutrophyculturemedium，andthetimeachievingtheculminatedpointoflogarithmicphasewasshortedalso，atthesametime，thestationaryphaselengthenedAftersecondaryculture，Streptococcusthermophiiusreac

9、heddirectlythelogphasehardlythroughthelagphase，andthenquicklyreachedthedeclinephaseThereweremorerelationsofthemetabolicregalitiesofthethreekindsofnutritivesubstanceswiththesuperconcentratedincubationprocessofStreptococcu

10、sthermophilus：themorethenumberofthebacteria，thequickerthemetaboling，whilethefewertheslowerthemetabolismMoreoverthereapparentlyexistedmorphologicchangesinthiscourse，andtheremaybeexistedtheapoptosis，whichcorrelatedwithbact