不同營養(yǎng)水平對蒙古綿羊肌內(nèi)脂肪沉積相關(guān)基因mRNA表達量的影響.pdf

1、分類號Q344.13UDC636學校代碼10129學號2010209008營養(yǎng)限制與補償對蒙古綿羊嫩度相關(guān)基因mRNA表達量的影響EffectofUndernutritionCompensationontheTendernessRelatedGenemRNAExpressioninSheep申請人：黃雅娟不同營養(yǎng)水平對蒙古綿羊肌內(nèi)脂肪沉積相關(guān)基因mRNA表達量的影響EffectofDifferentNutrientLevelsonthe

2、IMFRelatedGenemRNAExpressioninMongoliaSheep申請人：苗海明學科門類：工學學科專業(yè)：食品科學研究方向：食品質(zhì)量與安全指導(dǎo)教師：靳燁教授高愛武副教授論文提交日期：二〇一三年六月EffectofDifferentNutrientLevelsontheIMFRelatedGenemRNAExpressioninMongoliaSheepAbstractThisexperimentwasconducted

3、toresearchtheeffectsofnutritionrestrictioncompensationontheexpressionofIMFrelatedgenemeatqualityinMongoliansheep.EightyWuZhuMuQinsheep(averageweight14.72kg)wereassignedromlyto1of4groups:controlgroup(CG)restrictedgroupⅠ(R

4、GⅠ)restrictedgroupⅡ(RGⅡ)restrictedgroupⅢ(RGⅢ)respectively.Theresultswereshowedasfollow:1.ArealtimePCRmethodwhichwassuitablefdetectingthemRNAexpressionofIMFrelatedgenesinMongoliansheepwasestablished.Themethodhasgoodspecif

5、icityhighsensitivityquantitativeaccuracycanbeappliedtosubsequenttrialsquantitativedetectionofgeneexpressionanalysis.2.ThenutritionrestrictionpromotedtheexpressionofHFABP、FASHSLgene.LowenergylevelspromotedtheexpressionofL

6、PLPPARgenelowproteinlevelspromotedthePPARgeneexpressionbutinhibitedLPLgeneexpression.BothenergyproteinrestritionresultedinthesignificantdecreaseintheexpressionofLPLPPARgenes.Duringthenutritionalcompensationperiodtheexpre

7、ssionofHFABP、FASPPARgenesofRG3weresignificantlyhigherthanthoseofCGwhiletheexpressionofLPLHSLgenesofeachrestrictiongroupislowerthanthoseofCGindicatedthatthenutritioncompensationhaddifferenteffectsontheIMFrelatedgenes.3.At

8、theendoftherestrictionperiodthecrudefatcontentintramuscularfatcontentbackfatthicknesstheshearfcevaluesofRG3weresignificantlylowerthanthoseofCGexceptthepH45.Proteinrestrictionresultedinthesignificantincreaseinintramuscula

9、rfatcontent.AfternutritioncompensationtherewerenosignificantdifferencesbetweencrudefatcontentbackfatthicknesspH24waterlossrate.HoweverwhiletheintramuscularfatcontentofeachrestrictiongroupwerelowerthanthoseofCGshearfcevau

10、lesofRG1RG2weresignificantlyhigherthanthoseofCGRG3.Nutritionrestrictioncompensationhadnoeffectonmusclewaterholdingcapacitythroughthewholeexperimentperiod.Keywds:MongoliasheepCompensationIMFdepositionRealtimePCRMeatqualit