蘇尼特羔羊肌內(nèi)脂肪細(xì)胞分化相關(guān)因子和肌內(nèi)脂肪代謝關(guān)鍵酶基因表達(dá)發(fā)育規(guī)律的研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)博士學(xué)位論文蘇尼特羔羊肌內(nèi)脂肪細(xì)胞分化相關(guān)因子和肌內(nèi)脂肪代謝關(guān)鍵酶基因表達(dá)發(fā)育規(guī)律的研究姓名：姚焰礎(chǔ)申請學(xué)位級別：博士專業(yè)：動物營養(yǎng)與飼料科學(xué)指導(dǎo)教師：盧德勛20080401StudiesontheDevelopmentalExpressionoftheGenesInvolvedinIntramuscularAdipocyteDifferentiationandofKeyEnzymesInvolvedinIntramusc

2、ularFatMetabolisminSunitLambs纛bstrace16castratedSunitlambs稍也about10kgBWwereselectedandyardfed翻掩dietarynutrientlevelsforthelambswerearrangedaccordingtOtherecommendationsofKX涎8162004(Feedingstandardofmeatproducingsheepandg

3、oats)。Fouranimalswereslaughteredat10kg，20kg，30kgand40kgliveweightr6speetivelyandLongissimusDorsiWassampledforfurtheranalysisIntramuscularfatcontentandshearvalueweredeterminedtOstudythedevelopmentalpatternofintramuscularf

4、atandtherelationshipbetweenintramuscular紈andmeatqualityPPARandADDIhavebeenimplicatedaskeyregulatorsofadipocytedifferentiationwhileprc矗1isthemarkerofpreadipocyte。ItiswellknownthatACC，F(xiàn)AS，“毛andHSLarekeyenzymesinlipidmetabo

5、lism?；mRNAlevelsofthesegenes、weredeterminedtostudythedevelopmentalexpressionofthegenesinvolvedinintramuscularadipocytcdifferentiationandofkeyenzymesinvolvedinintramuscularfatmetabolisminSunitlambsbymeansofquantitativere

6、altimeRTPCRTheresultsshowedthat：1IntramuscularfatcontentandwaterholdingpercentageincreasedaslambsgrewwhilewatercontentandshearvaluedecreasedTherewasverysignificantnegativecorrelationbetweenshearvalueandintramuscularfatco

7、ntentofmutton鏟一◇983，p001)2ThereWasadecreasingtendencyofthedevelopmentalexpressionofPPARADDIandP羚lgenesfrom10to40kg，囂W髓lem_RNAlevelsofPPAR，ADDIandPref1in10and20kglambsweresignificantlyhigherthanthatin30and40kglambs(po05)。

8、TherewassignificantnegativecorrelationbetweenPref1mRNAabundanceandIMFcontent(嚴(yán)一O659，pO05)。3AslmnbsgrewthemRNAlevelsofACCandLpLgenesincreased]隱eirmRNAlevelsof30kglambswerehigherthanthatof10kgsheepwhilelowerthanthatof40kgl