一株高產(chǎn)酒精酵母菌種的選育與工藝優(yōu)化

1、天津大學(xué)碩士學(xué)位論文一株高產(chǎn)酒精酵母菌種的選育與工藝優(yōu)化姓名：陳龍泉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物工程指導(dǎo)教師：趙學(xué)明薛培儉20070701ABSTI認(rèn)CTYeaststrainZX19，whichiskeptbythestrainconservecenterofBBCA，wasstudiedastheinitialstraininaspectsofgrowthcurve；then，onthebaseofthesedatainthelog

2、arithmstage，yeastsweretreatedwithheat—shockandultraviolet，fultheTmore，廿leywerescreenedbyTTCplate，selectedbycultivatedinDusetube，againinshakingflasksAtlastahighyieldstrainZX一19—509withstablegeneticcharacterwasobtainedThis

3、researchstudiedthealcoholpatienceandhigh—concentrationsugarpatiencecharactersneresultis，strainZX19—509performsalittlehigheralcoholpailenceandcouldgrowin259／100mlsaccharificationsolutionwellSuchindexesofoptimumgrowthtempe

4、rature，optimumgrowthpH，seedstagesandinoculationratio，DEvalueofliquefacientsugarsolutionfermentationtemperature，andinitialpHvalue，concentrationofinorganicionandDEvalueofsaccharificationsolutionneoptimalresultis，theoptimum

5、growthtemperatureis30“CandtheoptimumpHis55，thebestseedstageandinoculationratioare22hand10％respectivelythetopwellDEvalueofliquefacientsugersolutionis20％，theoptimumfermentationtemperatureis32“CandthefittestinitialpHiS55，th

6、eDEvalueofsaccharificationsolutionbe96％EachaddingquantityofthefourconcentrationofinorganicionalsodeterminedWetookateston5001fermentorinlargebyusingtheobtainedstrainandoptimizedtechniques60hlater，theaveragealcoholyieldisu

7、pto127％(v／v)，andthetransformratiois451％，whichincreased07pointsinalcoholoutputcomparedtotheinitialstrainand24pointsintransformrationcomparedtothatof427％：Keywords：alcoholfermentation；Yeast；Highyieldstrains；Heatshock；ultrav