16659.血清饑餓誘導(dǎo)的周期同步化促進人類體細胞重編程的過程

1、中山大學(xué)博士后出站報告血清饑餓誘導(dǎo)的周期同步化促進人類體細胞重編程的過程Serumstarvationinducedcencyclesynchronizati伽facilitateshumansomaticcellsreprogramming評議委員會主席：委員：流動站(二級學(xué)科)：眼科學(xué)報告人：陳夢飛合作導(dǎo)師：葛堅、卓業(yè)鴻教授本研究受以下基金資助：國家基礎(chǔ)研究發(fā)展計劃(973計劃)：2007CB512207國家自然科學(xué)基金：30973

2、226中國博士后基金：200904608142012年12月廣州AbstractHumaninducedpluripotentStemcells(iPSCs)proVideaValuablemodelforregenerativemedicineandhumaIldiseaSeresearchTodate，howeVer，tllerepro伊amminge伍ciencyofhumaIladultcellsisstill10wRecent

3、studieshaVerevealedthatcellcycleisakeyparameterdriVingepigeneticreprogranuningtopluripotencyAsisweUknown，retroVimsessuchastheMoloneymurineleukemiavims(MoMLV)requireceUdiVisiontointe伊ateintothehostgenomeaIldreplicate，、Ⅳhe

4、reast11etargetprima巧cellsforrepro鏟ammingareamixtureofseVemlcell帥es誦tlldifrerentceUcyclerhynunsWhetherceUcyclesyncllronizationhaspotentiale疵ctonretrovirusinducedrepro伊跗姍inghasnotbeendetailedIntllisstudy，utilizing仃a11sient

5、semmstarVationinducedsynchronization，wedemonstratedmatstarvationgeneratedareversibleceUcycleanestandsyncllronouslyprogressedtllroughG2／MphaseaRerrelease，subst鋤tiallyimproVingretroViralinfectionef!ficiencyInterestingly，sy

6、ncluonizedhumaIldermalfibroblasts(HDF)arldadiposestemcells(ASC)exhibitedmorehomogenousepitheliaJmo印hologythannomalFBScontrolafberinfection，a11dt11eexpressionofepithelialmarkerssuchasEcadherina11dEpcamwerestronglyactiVate

7、dFuthe珊ore，syncllronizationtreatmentultimatelyimprovedNaIlogpositiVeclones，achieVeda15—20foldincrease7rheseresultssuggestedthatcencyclesyncllIonizationpromotesthemesenchymaltoepithelialtmsition(MET)aJldfacilitatesretroVi