嗅球成鞘細(xì)胞和幾丁質(zhì)植入治療大鼠坐骨神經(jīng)損傷的實(shí)驗(yàn)研究.pdf

1、該實(shí)驗(yàn)分離新生大鼠嗅球成鞘細(xì)胞(Olfactory Ensheathing Cells,OECs)進(jìn)行原代培養(yǎng),阿糖胞苷(arabinosyl cytosine,Ara-c)進(jìn)行純化,再用神經(jīng)生長因子受體(nerve growth factor receptor p75,p75NGFR)和膠質(zhì)纖維酸性蛋白(glial fiber acidic protein,GFAP)抗體進(jìn)行免疫組化鑒定.建立坐骨神經(jīng)離斷模型,硅膠管套接神經(jīng)斷端,管內(nèi)

2、局部植入培養(yǎng)的OECs細(xì)胞懸液和幾丁質(zhì)(chitin)溶液,并與SAL進(jìn)行對照.結(jié)果表明,OECs和幾丁質(zhì)能減少損傷側(cè)脊髓運(yùn)動神經(jīng)元的死亡率,降低脊髓運(yùn)動神經(jīng)元中ChE、ACP酶活性變化的幅度,并有利于新生神經(jīng)纖維髓鞘的形成,促進(jìn)損傷神經(jīng)電傳導(dǎo)功能、神經(jīng)元胞體逆行軸漿運(yùn)輸、坐骨神經(jīng)功能指數(shù)等的恢復(fù).因此,OECs和幾丁質(zhì)對外周神經(jīng)損傷所導(dǎo)致的脊髓神經(jīng)元死亡和退行性變有較好的保護(hù)作用,對外周神經(jīng)損傷后新生神經(jīng)功能的恢復(fù)具有積極的促進(jìn)作用.