人類神經(jīng)突起因子真核表達系統(tǒng)的構(gòu)建及功能的初步研究.pdf

1、石河子大學碩士學位論文人類神經(jīng)突起因子真核表達系統(tǒng)的構(gòu)建及功能的初步研究姓名：張峪涵申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：楊磊黃瑾20050501TheconstructionofeukaryoticexpressionsystemandprimaryfunctionalresearchofhumanneuritinPostgraduate：ZhangYuhanAdvisor：ProfessorYangleiAbstra

2、ct：Neurotrophicfactorsaregrowthfactorsorcytokinesthatareinduciblepolypeptidesandpermitintercellularcommunication，Neurotrophicfactorscallmodulatethedevelopmentofgrowth、maintenanceoffunction，anddifferentionofneuronalpopula

3、tionofnervoussystem，sotheyplayanimportantroleinsupportingtheinjurednervoussystemandneurodegerativedisorders，AnexplosionofinformationaboutneurotrophicfactorsissettingthestageforsignificantadvancesinneuraldiseasetherapyNeu

4、ritinwasanavelneurotrophicfactorwhichwasfirstreportedbyNediviasCandidatePlasticity—relatedgenesl5，onnaturein1993thenitwasnmnedbyIsraelscientistNaevein1997PreviousstudiesdemonstratedthatNeuritincaninduceneuritegrowth，prom

5、otetheoutgrowthandarborizationofneuriteBothBDNFandneuronalactivitycallactivetheexpressionofneuritinItsuggestthattheneuritinmaybeadownregulatedcofactorofBDNFandneuronalactivityNeuritinappliedforegroundinnerveregenerativef

6、ield，butthefunctionandmolecularmechanismofNeurifinisnotveryclearlysofarAtpresent，ahumanneuritineDNAwasclonedandthestructureandpotentialfunctionofitsproteinwaspredictedbybioinformaticsOnthebasisofpastworkweaimtoconstructe

7、ukaryoticexpressionsystemanddotheprimaryresearchofneuritinFirstly，thecodingsequenceofhumanneuritinwasclonedintopcDNA40eukaryoticexpressionvectorbyusinggeneticrecombinationtechniqueAfteranalysisbyrestrictionenzymedigestio

8、nandDNAsequencingtllerecombinantpcDNA40一neuritinplasmidwastransfectedintoPCI2cellsbyusingcationiclipid(Tfx20)FinallytheexpressionofneuritinwasdeterminedbyimmunocytochemistryTheresultshowedthatneuritinreadingframewascorre

9、ctTherecombinantplasmidcanexpressatmRNAlevelandproduceactiveNeuritinproteinineukaryoticcellsTheresearchappliedafoundmentalbasisforthefunotionandmechanismstudyofneuritininfutureKeywords：neuritin；eukaryoticexpressionvector