利用毒理與耐藥基因芯片對膠質(zhì)瘤化療后復(fù)發(fā)相關(guān)新候選基因的探索性研究.pdf

1、福建醫(yī)科大學(xué)碩士學(xué)位論文利用毒理與耐藥基因芯片對膠質(zhì)瘤化療后復(fù)發(fā)相關(guān)新候選基因的探索性研究姓名：胡勝利申請學(xué)位級別：碩士專業(yè)：外科學(xué)（神經(jīng)外科學(xué)）指導(dǎo)教師：林志雄楊麗娟20100401ExplorativeStudytoIdentifyNovelCandidateGenesRelatedtoPostchemotherapeuticGliomaRelapseUsingaOligoToxicology＆DrugResistanceMicro

2、arrayAbstractObjective：Tostudytheexpressionoftoxicology&drugresistancegeneandmolecularrelatedtostressresponseinpreandpostchemotherapeuticgliomaandtoexplorenovelcandidategenesrelatedtopostchemotherapeuticgliomarelapseMeth

3、ods：EctopiclysubcutaneousgliomatransplantationmodelsinnudemicewereestablishedbyinjectionofhumangliomacelllineU251cellsTumorbearednudemiceweretreatedbytheintraperitonealinjectionofcisplatin(atthedoseof5mg／kg／dfor45dayscon

4、tinuously)，chemotherapeuticandrecurrentgliomamodelafterchemotherapywereestablishedGliomaswereharvestedasagroupofgliomasafterchemotherapyonthenextdayofcompletionofchemotherapy；about1weekafterchemotherapygliomaswereharvest

5、edaSagroupofsmallestgliomaafterchemotherapywhengliomassignificantlyreducedbutdidnotgrowagain；about10tol4daysafterchemotherapygliomaswereharvestedasagroupofrecurrencegliomasafterchemotherapywhengliomasrecurredrapidlyExpre

6、ssionoftoxicology&drugresistancegeneandmolecularrelatedtostressresponseinpreandpostchemotherapeuticgliomaweredetectedusingoligotoxicology&drugresistancemicroarraysDataingenechipWasanalyzedbystatisticalsoflrwareSPSS15ORes

7、ults：ExpressionofABCCIincreasedgraduallyingliomasafterchemotherapyExpressionofABCC5andBCL2increasedingliomasatcompletionofchemotherapyandsignificantlydecreasedwhengliomasWassmallestandincreasedobviouslywhengliomasrecurre

8、dExpressionofHSPB2increasedingliomasafterchemotherapyitsexpressionWashighestwhengliomaswassmallestanddecreasedwhengliomasrecurredExpressionofNFKB1、NFKBIAandBAGIdecreasedingliomasatcompletionofchemotherapyandincreasedgrad