干擾素gamma及腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體治療卵巢癌大鼠的體內(nèi)研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文干擾素gamma及腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體治療卵巢癌大鼠的體內(nèi)研究姓名：陳婧申請學(xué)位級別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：郝敏20090416山西醫(yī)科大學(xué)碩士學(xué)位論文THERESEARCHONTHECOMBINATIONOFIFNYWITHTRAILT0OVARIANCANCERRArSINⅥVOAbstractOvariancancerisoneofcommonfemalereproductivesystemt

2、umorsThemorbidityisjustlowerthanuterinecervixcancerandendometrialcancerThemortalityisthehighestofthegynecologictumorsAlthoughtheleveloftherapyhasbeenimproved，ThefiveyearsurvivalisstilllowerThereforetheresearchofnewtherap

3、ywaybecomenewresearchcenterTheresearchonthecombinationofIFN—YwithTRAILCaninhibitthegrowthofovariancancercellsinvitroTheexperimentinvestigatestheeffectofinterferon—gammaandTRAILontheovariancancerratsbyconstructingovarianc

4、ancerratsObjective：ToinvestigatetheeffectsandthemechanismofInterferongamma(IFN—Y)andTumornecrosisfactorrelatedapoptosisinducingligand(TRAIL)ontheovariancancerratsinvivoMethods：Tochoose24healthyinbreedinglinefemaleWistarr

5、ats，selectscapularregiontoconstructsubcutaneousovariancancermodel。Aftermodelconstruction，fractionateinto4grouprandomly，groupAisblank，groupBisTRAILinterventiongroup，groupCisIFNYinterventiongroup，groupDiscombinationofTRAIL

6、withIFNYinterventiongroupInterventionbyinjectingdifferentinterventingfactorintotumorregionevery3daysExaminingtheapoptosisoftumorcellsbyflowcytometry(FCM)Results：Theapoptosisrateof4groupare512％，2121％，19。62％and492％Theapopt

7、osisrateofgroupAandgroupBisevidentlydifferent(P005)TheapoptosisrateofgroupAandgroupCisevidentlydifferent(P005)ComparedwithgroupBandgroupC，theapoptosisrateofgroupDisevidentlydifferent(P005)Conclusion：Thecombinationofthetw

8、otreatmentsinovariancancerratsinducedsignificantlyhigherapoptosisthanexposuretoTRAILorIFN—yalone，theymayoffersynergisticbenefitswhenusedconcurrentlyKeywordsTumornecrosisfactors；interferongamma；ovariancancer；cellapoptosis