腫瘤宿主T和NK細(xì)胞ξ鏈水平下調(diào)機(jī)制及其逆轉(zhuǎn)的初步研究.pdf

1、中國協(xié)和醫(yī)科大學(xué)中國醫(yī)學(xué)科學(xué)院碩士學(xué)位論文腫瘤宿主T和NK細(xì)胞ξ鏈水平下調(diào)機(jī)制及其逆轉(zhuǎn)的初步研究姓名：韓揚(yáng)申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：免疫學(xué)指導(dǎo)教師：張叔人2002.5.1√。中國協(xié)和醫(yī)科大學(xué)碩L學(xué)位論文Studyondownregulationmechanismofzeta(曲chaininTandNKcellsoftumorbearinghostandreversemethodsAbstractTandNKcellsarethemain

2、effectorcellsonclinicaltumorimmunotherapyEchainjsthepivotalsignaltransductionmoleculeonlyexpressedinTandNKcells，whichplaysanimportantroleinTandNKcellactivationHowever芒chaininthemostofthecancerpatientTandNKcellswas10wexpr

3、essedorlostItcouldbeabarrierforimmunotherapyUptodate，themechanismcannotbeunderstoodclearlyThisisthefirstresearchontherelationshipbetweentumorand芒chain，themechanismfordownregulationof芒chainand芒chain—protectedmethodsatthes

4、ametimeTheEchainexpressionleveldetectedbyflowcytometryassaysinperipheralbloodTandNKcellsin80casesofrenalandbladdercancerpatientswas80％lowerthannormalcontrolsTheresultsshowedthatthedown—regulateddegreesof鼉chainweresignifi

5、cantlydependentontumorprogression鼉chaincanbedownregulatedbyLPS—stimulatedmacrophageorPGE2／nvitro，butnotbyIL廠10andIGFIItwasprovedthatlOWlevelof芒chaininthemousemodelinducedbytumorcanbepartiallyreversedbytreatmentwiththetra

6、ceelementSe，SLCgeneorChineseherbBH芒chaingenewasclonedfromhumanperipheralbloodandtheproteinwassuccessfullyexpressedusinginsect／baculovirusexpressionsystemTheresultsdemonstratedthat芒chainlevelofTandNKcellscouldbeasbiologic

7、almarkertoevaluatedevelopmentoftumorandimmunestateinthehostThesuppressiveeffectofPGE2ontchainexpressioniSenrichmentofmechanismintumorimmunology丁reatmentwiththetraceelementSeSLCgeneorChineseherbBHiSanewideafortumortherapy