太白銀蓮花活性成分研究.pdf

1、太白銀蓮花(Anemone taipaiensis)為陜西省特有的銀蓮花屬植物，已有的研究表明從銀蓮花屬植物中分離出的三萜皂苷類成分有確定的抗腫瘤、抗炎等生物活性，但有關(guān)該植物的研究尚未見報(bào)道。
　　本文利用硅膠柱色譜、Sephadex LH-20柱色譜、反相ODS柱色譜及HPLC等分離手段從太白銀蓮花根莖70％乙醇提取物中分離得到了30個(gè)化合物，并通過(guò)化學(xué)和光譜學(xué)方法鑒定了化合物的結(jié)構(gòu)，包含10個(gè)新化合物，1個(gè)新天然產(chǎn)物以及19

2、個(gè)已知化合物，其中27個(gè)為齊墩果烷型三萜皂苷類化合物。分別為：β-谷甾醇 (1), 3b-O-(a-L-arabinopyranosyl}oleanolic acid (2),β-胡蘿卜苷 (3), 翻白葉苷 A (4), 刺楸皂苷 A (5), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl}oleanolic acid (6),

3、 sapindoside B (7), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl} oleanolic acid 28-O-b-D-glucopyranoside (8), cussonside B (9), 3b-O-{a-L-rhamnopyranosyl-(1→2)-[b-D-glucopyranosyl-(1→4)]-

4、a-L-arabinopyranosyl}oleanolic acid (10), 白頭翁皂苷 D (11), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rhamnopyranosyl-(1→2)-[b-D-glucopyranos-yl-(1→4)]-a-L-arabinopyranosyl}hederagenin (12), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rhamnop

5、yranosyl-(1→2)-[b-D-glucopyranosyl-(1→4)]-a-L-arabin-opyranosyl} oleanolic acid (13), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rham-nopyranosyl-(1→2)-[b-D-glucopyranosyl-(1→4)-b-D-glucopyranosyl-(1→4)]-a-L-arabinopyranosyl} ole

6、anolic acid (14), sieboldianoside B (15), cernusoide C (16), 紅毛七皂苷 D (17), 3b-O-{b-D-glucopyranosyl-(1→4)-a-L-arabinop-yranosyl}siaresinolic acid 28-O-{a-L-rhamnopyranosyl-(1→4)-b-D-glucopyra-nosyl-(1→6)-b-D-glucopyranos

7、ide} (18), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl}siaresinolic acid 28-O-{a-L-rhamnopyranosyl-(1→4)-b-D-glucopyranosyl-(1→6)-b-D-glucopyranoside}(19), hederacolchiside F (20), leonto

8、side (21), sieboldianoside A (22), 刺楸皂苷 B (23), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rhamnopyranosyl-(1→2)-[b-D-glucopyranosyl-(1→4)]-a-L-arabinopyranosyl} oleanolic acid 28-O-{a-L-rhamnopyranosyl-(1→4)-b-D-glucopyranosyl-(

9、1→6)-b-D-glucopyranoside}(24), hederacolchiside E (25), 3b-O-{b-D-xylopyranosyl-(1→3)-a-L-rhamnopy-ranosyl-(1→2)-[b-D-glucopyranosyl-(1→4)]-a-L-arabinopyranosyl} siaresino-lic acid 28-O-{a-L-rhamnopyranosyl-(1→4)-b-D-glu

10、copyranosyl-(1→6)-b-D-glucopyranoside}(26), 3b-O-{a-L-rhamnopyranosyl-(12)-[b-D-glucopyrano-syl(14)]-a-L-arabinopyranosyl} siaresinolic acid 28-O-{a-L-rhamnopyrano-syl-(14)-b-D-glucopyranosyl-(1?6)-b-D-glucopyranoside}(2

11、7), 3b-O-{b-D-xylopyranosyl-(13)-a-L-rhamnopyranosyl-(12)-[b-D-glucopyranosyl-(14)-b-D-glucopyranosyl-(1?4)]-a-L-arabinopyranosyl} hederagenin 28-O-{a-L-rhamnopyranosyl-(14)-b-D-glucopyranosyl-(1?6)-b-D-glucopyranoside}(

12、28), 3b-O-{b-D-glucopyranosyl-(1?4)-b-D-glucopyraosyl-(1?4)-[a-L-rham-nopyranosyl-(12)]-a-L-arabinopyranosyl} hederagenin 28-O-{a-L-rhamn-opyranosyl-(1?4)-b-D-glucopyranosyl-(16)-b-D-glucopyranoside} (29), 3b-O-{b-D-xylo