食源性單核增生性李斯特氏菌分布及其內(nèi)化素基因分布研究.pdf

1、河北農(nóng)業(yè)大學碩士學位論文食源性單核增生性李斯特氏菌分布及其內(nèi)化素基因分布研究姓名：胡楊峰申請學位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導教師：賈英民20090612ThestudyofDistributionInternalinGenesofListeriamonocytogenesamongfoodsamplesAuth:HuYangfengSupervis:JiaYingminMaj:PrimaryProductProcessing

2、StageEngineeringAbstractListeriamonocytogenesisakindofimptantzoonoticdiseasepathogenswhichcancausepersonsvariouslivestocksfowlsseriousdiseases.Listeriamonocytogeneshasbeenconsideredzoonoticdiseasepathogensinalloverthewld

3、since1940s.FromthepublichealthpointofviewListeriamonocytogenesasoneofthemostimptantfoodbnepathogenithasbeenpaidmuchmeattentionofthepeoplesince1990s.Manyexpertsscholarsathomeabroadstudythebiologicalacteristicsdetectiontec

4、hnologypathogenicitygenevirulencefactsepidemiologicalofListeriamonocytogenes.ThispaperisolatedidentifiedListeriamonocytogenesbythefirststepofthephysiologicbiochemicaltestsfrom1627samples.1627romsamplesfrom9stsofquickfroz

5、enfoodaquaticproductsreshmeatmeatprotuctsfruitvegetablesbeanproductrawmilksaltedproductwerecollectedfromsupermarketspeasanttrademartsomefaecesofanimalperson.ThetotaldetectablerateofListeriamonocytogeneswas14.5%.Theresult

6、sshowedthatthedetectablerateofListeriamonocytogeneswerehigher.ItexpressedthatpollutionlevelofListeriamonocytogenesinBaodingcircumjacentareawastaller.StudyingdistributingstatusofListeriamonocytogenesitwasdiscoveredthatthe

7、ablerateofListeriamonocytogenesinreshmeatsampleswasthehighest(27.4%).Thesecondratewas24.7%insaltedproduct.Itindicatedthattheprobabilityofthisbacteriumtopollutereshmeatsaltedproductwerehighweshouldattachimptancetoqualityp

8、roblemoftheseproductstoavoidbreakingoutoffoodbnediseases.Itwasveryimptanttodefendthepathogen.AtpresentListeriamonocytogenesinternalingenescanbedividedinto9types(inlAinlBinlCinlC2DinlDinlEinlFinlGinlH).Accdingtoannouncedl

9、iteraturesinlAinlBwereinallofListeriamonocytogenesstrains.inlCinlC2DinlDinlEinlFinlGinlHwereincertainofListeriamonocytogenesstrains.OwingtoidentifyingthemtogenuscertainlythesestrainswereidentifiedbyPCRwithprimersofinlAin

10、lBinlCinlC2DinlDinlEinlFinlGinlH.TheywerespecialpathogenicgenesofL.monocytogenestheseprimersweresynthesizedaccdingtoprimersequencesinannouncedliteratures.Identifiedinternalingeneof236strainswhichhadbeenisolated2wereident