鹽酸小檗堿生物可降解緩釋體系的制備和性能研究.pdf

1、廈門大學碩士學位論文鹽酸小檗堿生物可降解緩釋體系的制備和性能研究姓名：江宇良申請學位級別：碩士專業(yè)：生物化工指導教師：劉慶林20090601AbstractAbstractThepresentworkusedstarchalginateandcarboxymethylchitosan(CMC)topreparestarch／alginateinterpenetratingpolymernetwork(IPN)microspheresan

2、dalginatemicrosphere／CMChydrogeldrugdeliverysystem(DDS)Modifiedemulsification／gelationmethodwasusedtoprepareblendmicrospheresMicroscopyandSEMwereusedtoobservethemorphologyThemicrosphereske：ptsphericalandwelldispersedinwa

3、terThemicrospheresCanbasicallykeepsphericalwitlllittledeformationafterfreezedryingwhichcouldbeobservedbySEMThesizedistributionWasmainlyaffectedbypreparationconditionsespeciallystirringspeedandO／WratioIncreasingstirringsp

4、eedanddecreasingO／WratiowouldcauseadecreaseinsizeanduniformsizedistributionWefoundthat2：1WasaproperW／atioPolymerconcentrationalsoaffectedsizeandsizedistributionFTIRprovedthatthetwopolymerswerewellmixedWetookberberinehydr

5、ochlorideasamodeldrugtobeencapsulatedbytheblendmicrospheresHighestencapsulationefficiencyofmorethan80％WasachievedbyusingproperconditionsConfocallaserscanningmicroscopy(CLSM)Wasusedtoobservethedrugdistributioninmicrospher

6、esDrugdistributionwasuniformDrugreleaseperformanceWasstudiedinbothpH74phosphatebuffersolution(PBS)andpH12hydrochlorideacidsolution(HAS)at37士O5。Cand50r／mminThedrugconcentrationwasdeterminedbyUVat345nlnItWasfoundthatreleas

7、einPBSWasfastandaconsiderableburstreleasehappenedOnthecontraryadelayedreleaseWasnotedinHASThereleasedatawerefitted、ⅣitllseveralclassicmodelsItWasfoundthatafirst—ordermodelfittedbestInordertogetmoredelayedreleaseanddecrea

8、seburstrelease，newDDSofalginatemicrosphere／CMChydrogelWaspreparedThesurfacemorphologyWasobserves、析tllmetallographicmicroscopeandSEMMicrospherescouldbeseentobedistributedinhydrogelMoremicrospherescouldbeseeninDDSwithmorem

9、icrospheresaddedMechanicaltestprovedthatDDShadbettercompressivestrengththanpurehydrogelButincreaseofmicrosphereswouldcausealittledecreaseofcompressivestrengthReleaseperformancewasstudiedinpH12HAS、pH74PBSandpH63salinesolu