羧甲基殼多糖對皮膚成纖維細胞生長的影響及生物組織相容性研究.pdf

1、青島海洋大學碩士學位論文羧甲基殼多糖對皮膚成纖維細胞生長的影響及生物組織相容性研究姓名：王真申請學位級別：碩士專業(yè)：海洋生物指導教師：陳西廣2000.6.1童璺塑堂奎蘭雯主堂墊望蘭塑里苧查墨壁墅鏖壁盛塹絲塑墮竺蘭塑墅墮璺生塑望塑塑查壁塑!簍一TheeffectofcarboxymethylchitosanonthegrowthofskinfibroblastsanditsbioIogicalcompatibiIit)rCarboxyme

2、tllylchitosan(CM—CH)isawate卜solublederiVatiVeofchitos舡1Usingmeskintissueofyellowpeople，wesuccessfullyculturedfibroblastsofnormaladultskin，fbtalskinandkeloidinvitrDandwestudiedmeefrectofCMCHonmeproliferationof也esefibrobla

3、Stsandc01lagenmetabolismTheresultsdemons仃ated協(xié)atCM—CHpromotedproliferationofnolmalskinfibroblastsandmarkedlyinhibitedthegrowChofkeloidderivedflbroblastsCMCHmarkedlyreducedt11esynmesisofcollagenofkeloidfibroblastsTheresul

4、tsofmrtherstudydemonstratedCMCHirIlibitedthesyntIlesisoftypeIcollagenaIlddidnotch鋤gemesynmesisoftypeIIIcollagenforkeloidfibmblastCM—CHdidnotmarkedlyaffectthesynthesisofcollageninno哪alskin助roblastsCM—CHmembranewasprepared

5、toinVestigatetheaffinitybetweenCM—CHaJldserumproteinsTheresultssuggestedtllatCM—CHfilmcouldremarkablyadsorbserumpmteinsoncertainpHconditionsandtheadso印tionhadselectiVityFibroblastscouldadhereont11efilmofCM—CHandprolifera

6、tequicklyforalongtimeTheseresultsshowedCMCHhadgoodbiologicalcompatibili妙HemolysisandcelltoxicityexperimentsshowedthatCM—CHdidnotexpresstoxicityt0cellsKeywords：carboxymethyl—chitosall(CM—CH)肋roblastkeloidproliferationcoll