高產(chǎn)瓊膠酶菌株的選育和產(chǎn)酶的研究.pdf

1、中國海洋大學碩士學位論文高產(chǎn)瓊膠酶菌株的選育和產(chǎn)酶的研究姓名：湯海青申請學位級別：碩士專業(yè)：水產(chǎn)品加工及貯藏工程指導教師：管斌20040601StudysonthebreedingofagaraseoverproductionstrainandtheconditionsofagaraseproductionAbstractAnagardecomposingstrainwasisolatedfromsomekindsofredagarin

2、thecostalwaterinQingdaoandpresumptivelyclassifiedwithinthegenusPseudomonas1tgrowsatanoptimUlTItemperatureof20～40“CandapHof50～90NaCIorseawaterarenotstrictlyrequiredforgrowth，but2O～40％NaClishelpfulBesidesag罵PseudomonasS仕ai

3、nAT22carlalsodegradecarboh)，dratessuchasalginateandstarchThisoriginalstrainWastreatdrepeatedly、^，itllvariousmutagens(UVEMS)inscmemnghighproducerofagarse，andthemutantwithhi【ghandstableyieldofagaraseisselectedafterrepeated

4、naturalselection，Withthesinglefhctortestandrelationshiptest。theoptimalculturemediaforAT22s廿ainproducingagaraseisdecidedas：agarO3％，(NI％)2S0405％，peptone03％，NaCl3O％，KH2P0401％，MgCl201％；andtheoptimalcultureconditionssuchasinc

5、ubatingtemperature，mediumpH，rotationrateandamountofinoculationare24“(2，70，120rpmand1％respectively300mLEr]enmeyerflaskcontaining100mLmediumTheagaraseactivityoffermentedbrothis800U／mLwhenPseudomonasspAT22isincubatedinErlen

6、meyerflaskundertheoptimalconditionsat3648hTheagarasefromPseudomonasspAT22isinducibleandsecretesextracellularlyExazymeproductionisreducedifmonosaccharideandoligosaccharide，suchasglucose，galactoseoracidhydrolysisproducts，a

7、readdedtothemudium，ItsoptimaltemperatureandpHforhydrolyzingagarare40“CandpH70respectivelyAndtheagaraseisstableinthepHrangeof60～70，theoptimalconcentrationofsubstrateis10～12％，additionofCa2willgreatlypromotetheenzymeactivit