化癥膠囊誘導前列腺癌細胞凋亡的實驗研究.pdf

1、湖北中醫(yī)學院碩士學位論文化癥膠囊誘導前列腺癌細胞凋亡的實驗研究姓名：王軍申請學位級別：碩士專業(yè)：中醫(yī)臨床基礎指導教師：趙映前2003.6.1StudyOntheApoptosisofProstateCancerCellPc3InducedbyHuazhengJiaonangMastercandidate：WangIunSupervisor：ZhaoYingqianABSTRACTobjective：Toinvestigateinduct

2、ionofapoptosisinhuazhengjiaonangtreatedpc一3celllineMethods：MTTassaywasusedtodetectthehalfinhibitionconcentration(ICso)ofhuazhengjiaonangonpc3cellline，thenthehuazhengjiaonangwithappropriateconcentrationWaschosentotreatpc一

3、3cellsforacertaintimeMorphologicalchangesofapoptotiecellswereinvestigatedThepc一3ceilsapoptosisinducedbyhuazhengjiaonangwasinvestigatedbyusingaseriesofquantitativeandqualitativemethodsDNAfragmentationWasanalyzedbyagaroseg

4、elelectrophoresisAdditionallythenumberofapoptoticcellsandtheDNAcontentwerequantitativelyassessedbyflowcytometry(FCM)Results：Thepc3cellstreatedbyhuazhengiiaonangrevealedmanycharacteristicmorphologicalfeaturesconsistentwit

5、hapoptosis，suchasthecellgrowthinhibition，theproliferationspeeddecrease，thecellsroundedupandshrtmkenThetransmissionelectronmicroscopicanalysisoftheuttrastructuralchangesoccurringinpe3cellstreatedbyhuazhengjiaonangrevealed

6、somemorphologicalchangeswhichincludedalteredcytosolicdensitypronouncedvacuoleformation，cllromatincondensationandnuclearfragmentationatthemarginofnuclearenvelopethedilationofcytoplasmicorgandiessuchasmitochondriaandroughe

7、ndoplasmicreticulum，andtheapoptoticbodyformationAtthesflmetime，thepc一3cellswereinducedthespecificbiochemicalchangesconsistentwithapoptosisbyhuazhengjiaonangForexample，theagarosegelelectrophoresisofextractedgenomicDNAfrom

8、huazhengiiaonangtreatedcellsshowedthefragmentationofgenomicDNAintoladderingpatternsTheDNAcontentanalysisofFCMshowedpre—GIcellsIntheexperimentrange，theapoptosisinducingeffectofhuazhengiiaonangwasdose—dependentConclusion：H