藏紅花素對(duì)急性淋巴細(xì)胞白血病患兒淋巴細(xì)胞功能的影響.pdf

1、藏紅花素對(duì)急性淋巴細(xì)胞白血病患兒淋巴細(xì)胞功能的影響摘要目的本研究旨在探討藏紅花素對(duì)急性淋巴細(xì)胞白血病患兒淋巴細(xì)胞體外增殖及功能的影響。方法采用FicollHypaque法分離8例急性淋巴細(xì)胞白血病完全緩解半年以上l~10歲兒童外周血單個(gè)核細(xì)胞，分為對(duì)照組、植物血凝素組(PHA終濃度10pg／m1)和四個(gè)實(shí)驗(yàn)組，即：藏紅花素終濃度分別為0625mg／ml(A組)、125mg／ml(B組)、25mg／ml(C組)、5mg／ml(D組)，各實(shí)

2、驗(yàn)組均加入終濃度為10pg／ml的PHA。各組終體積均為lml。恒溫箱中培養(yǎng)三天。噻唑蘭(MTT)法檢測(cè)各組淋巴細(xì)胞增殖活性變化。ELISA法檢測(cè)各組上清液中自介素一2(IL2)和白介素一4(IL一4)的水平。流式細(xì)胞術(shù)檢測(cè)對(duì)照組、C組和D組中CD4T細(xì)胞和CD8T細(xì)胞的百分率及CD4／CD8比值。結(jié)果①藏紅花素對(duì)淋巴細(xì)胞增殖指數(shù)的影響：對(duì)照組淋巴細(xì)胞增殖指數(shù)為(098004)：植物血凝素組為(128O07)；實(shí)驗(yàn)A、B、C、D組分別為

3、：(136008)、(164015)、(176020)、(202010)；植物血凝素組，實(shí)驗(yàn)A、B、C、D組均明顯高于對(duì)照組(F=57775，pO01)，實(shí)驗(yàn)B、C、D組明顯高于植物血凝素組(pO05)；各實(shí)驗(yàn)組間比較，隨藏紅花素濃度的增加，淋巴細(xì)胞增殖指數(shù)升高，差異具有顯著的統(tǒng)計(jì)學(xué)意義(P005)。植物血凝素組與實(shí)驗(yàn)A組比較差異無(wú)統(tǒng)計(jì)學(xué)意義。②IL2濃度：對(duì)照組IL一2含量為(958349)pg／ml：植物血凝素組為(2201704)

4、pg／ml；實(shí)驗(yàn)A、B、C、D組分別為：(2490770)pg／ml、(2544771)pg／ml、(3593941)pg／ml、(4419807)pg／ml；植物血凝素組、實(shí)驗(yàn)A、B、C、D組明顯均高于對(duì)照組(F=2809，PO01)；實(shí)驗(yàn)C、D組高于植物血凝素組及實(shí)驗(yàn)A、B組(PO05)；實(shí)驗(yàn)D組高于實(shí)驗(yàn)C組(PO05)。植物血凝素組與實(shí)驗(yàn)A、B組比較，實(shí)驗(yàn)A組與B組比較，差異無(wú)統(tǒng)計(jì)學(xué)意義。③IL4濃度：對(duì)照組IL4含量為(1255

5、134)pg／ml；植物血凝素組為(1850071)pg／ml；實(shí)驗(yàn)A、B、C、D組分別為：(1863152)pg／ml、(2489151)pg／ml、(2761087)pg／ml、(2867086)pg／ml；植物血凝素組、實(shí)驗(yàn)A、B、C、D組明顯高于對(duì)照組(F=33836，PO01)；實(shí)驗(yàn)B、C、D組高于植物血凝素TheeffectofcrocinonTlymphocytesdevidedfromPeripheralbloodofc

6、hildrenwithacutelymphocyticleukemiaforcompleteremissioninvitroAbstractObjectiveTheeffectsofcrocinonCellproliferationcapacityandcytokinereleaseofstimulatedperipheralbloodmononuclearcellsbyph”ohema991utinin(PHA)wereexamine

7、dMethodsThemonomuclesrcellswereisolatedfromperipheralbloodofchildrenwithacutelymphocyticleukemiaforcompleteremissionbythemethodsofFicoll—Hypaqueanddividedintoonecontrolgroup、phytohemagglutinin(PHA)groupandfourexperimenta

8、lgroups(A—D)，whichpropotionalcrocinfinalconcentrationwereO625，125，25and5mg／m1After3daysculturation，theeffectsoffourconcentrationsofcrocin，phytohemagglutinin，andphosphatebufferedsolutiononcellproliferationandproductionofc

9、ytokines，includinginterleukin(IL)一2andIL一4wereevaluatedbyMTTassaytheIL一2ELISAkitandtheIL4ELISAkitUsingtheflowcytometrytheTlymphocytesubsetdistributionweredeteciedResultIncellsstimulatedwithPHA，differentconcentrations(fro

10、m125mg／mlto5mg／m1)ofcrocinsignificantlypromotedcellproliferationoflymphocytesandtheproliferationindexgraduallyincreasedwhenthecrocinconcentrationincreased，thedifferenceamongthemweresignificant(P005)Highconcentrationsofth