基因組改組技術(shù)選育嗜果糖、耐so2葡萄酒酵母畢業(yè)論文

1、LANZHOU UNIVERSITY OF TECHNOLOGY畢業(yè)設(shè)計(jì)(論文)題 目 基因組改組技術(shù)選育嗜果糖、耐 SO2葡萄酒酵母學(xué)生姓名學(xué) 號(hào)專業(yè)班級(jí)指導(dǎo)教師學(xué) 院答辯日期2AbstractBy UV induced mutagenesis and chemical mutagenesis methods of wine yeast strain C-1, C-2,C-3 and C-4,and screening on nutr

2、ient medium containing high concentrations of SO2 and fructose, weobtained 2 SO2 tolerance improved strains C-2-4 and C-4-2 and two fructose utilization increasingstrains C-1-8 and C-3-1. We made use of genome shuffing t

3、o the library of 4 generate improved strainsin this work.We shuffed twice these improved strain by protoplast fusion and finally obtained 5 strainswith higher SO2 tolerance and addicted to fructose. The SO2 tolerance of

4、the first round of thereconstituted strain A-2 improved 59.7% compared with the C-4-2, A-7 features the use of fructoseincreased by 20% compared with C-1-8. The SO2 tolerance of the second round of Genome Shufflingstrain

5、 B-1 has a 14% increase compared with the C-4-2, and the use of fructose characteristicsincreased by 34 % compared with C-1-8.Key words:Wine yeast; Genome Shuffling; Resistance to SO2; Addicted to fructose; Protoplastfus