芝麻抗莖點枯病鑒定及抗病基因同源序列的克隆.pdf

1、鄭州大學碩士學位論文芝麻抗莖點枯病鑒定及抗病基因同源序列的克隆姓名：高樹廣申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：田保明；劉紅彥201105AbstractAbstractSesame(SesamumindicumL)belongstoPedaliaceaeSesamumcultivatedwidelyinsubtropicsandtemperatezonesSesameistheoldestoilseedcrop，richine

2、dibleproteins，vitamins，fattyacidsaswellasaminoacidsSesamecharcoalrotcausedbyMacrophominaphaseolina(Tassi)GoidisoneoftheseverestfungaldiseasesThispathogenhasanextensivehostrange，astrongresistancetoadverseconditionsinaddit

3、iontotheemergingoffungicideresistantstrains，whichhasanadverseimpactonyield，qualityandmechanizationInrecentyears，environmentpollution，pesticideresiduesbesidesecologicalproblemarehighfrequencyduetotheincreasingdosagesoffun

4、gicideAlltheserequirepathologiststosearchforenvironmentally—friendlymeasurestocontrolsesamecharcoalrot，ItisprovedthatUSeofcropresistanceiSaneconomical，safeandvalidmeantocontrolcropdiseasesthroughlongtermpracticeofcropdis

5、easecontr01OneoftheresearchpurposeswastoselectresistantresourcesfortheresistancebreedingandproductionapplicationthroughidentifingsesameresistancetoMacrophominaphaseolina(Tassi)GoidinseedingstageandadultTheotherpurposewas

6、toisolateresistancegeneanalogstolaythefoundationforthecloneofresistancegeneorbeapplicabletomolecularmarkassistedbreedingThemainresearchconclusionsareasfollows：1FivestrainswereisolatedfromsesamecharcoaIrotplantswhichcamef

7、romdifferentregionsinHenanprovinceTheyallwereconfirmedasMacrophominaphaseolina(Tassi)Goidaccordingtosurveyingthemorphologyofcolonyreinoculationandidentificationaswellasmoleculardetection；2Thebeststeriletechniquebycompari

8、ngthedifferentsterilantseffectwasthat2％NaCIOprocesssesameseedsfortwentyminites，aRerthatsterilewaterwashedthreetimes；3ThisstudyevaluatedtwenteensesamevarietiesseedingresistancetofiveMacrophominaphaseolina(Tassi)Goidstrain