豬CAST基因多態(tài)性與肉質(zhì)性狀相關(guān)性的研究.pdf

1、摘要摘要本研究利用PCRRFLP技術(shù)對杜洛克(50頭)，原種榮昌豬(65頭)，內(nèi)江豬(38頭)、新榮昌豬I系〔72頭)的CAST基因進(jìn)行多態(tài)分析，并就限制性酶切位點多態(tài)度性與嫩度、肉色、滴水損失、失水率、大理石紋和肌內(nèi)脂肪含量等肉質(zhì)性狀之間的相關(guān)性進(jìn)行了研究，旨在尋找特異性分子標(biāo)記，為CAST基因作為肉質(zhì)性狀，特別是嫩度的輔助選擇標(biāo)記分子奠定基礎(chǔ)。結(jié)果如下:1三個豬品種的遺傳多樣性(1)杜洛克、內(nèi)江豬、榮昌豬在HinfiMsplRsal

2、均存在多態(tài)性，基因頻率處不平衡狀態(tài)。(2)檢驗表明:杜洛克、內(nèi)江豬、榮昌豬的優(yōu)勢基因型分別為ABCCEFAACCEEAACCFF，并且差異極顯著印0.01):如將杜洛克的優(yōu)勢基因型增至AACCFFBBDDEEABCDEF，內(nèi)江豬的優(yōu)勢基因型增至AACCEEABCDEE，此時三個品種的優(yōu)勢基因型在95%程度上與品種相關(guān)。(3)在對杜洛克豬CAST基因進(jìn)行Hinfl酶切時發(fā)現(xiàn)，產(chǎn)生一條大小約為120bp的非特異帶，新酶切位點產(chǎn)生的原因，雖然

3、有待進(jìn)一步研究，但由于MsplRsal酶切不發(fā)生變化，因此，可推測由于引種，環(huán)境的差異導(dǎo)致該基因點突變的可能性最大。2新榮昌豬I系CAST基因多態(tài)性與肉質(zhì)性狀相關(guān)性本實驗借助Popgen32和SPSS軟件，對72頭豬CAST基因多態(tài)性基因型與嫩度和肉色等肉質(zhì)性狀進(jìn)行相關(guān)性分析后發(fā)現(xiàn):(l)單酶切位點由F檢驗知:CASTHinfl酶切位點各荃因型的大理石紋值差異顯著因此，可作為大理石紋的候選基因。此時可只排除CASTHintl酶切含BB型

4、豬。F檢驗發(fā)現(xiàn):肌脂含量工MP(%)在V1spI酶切位點，各基因型間差異極顯著(p0.01)因此.ifspI酶切位點可作為肌脂含量(IMP)的遺傳標(biāo)記。可通過增加DD基因型頻率來提高IMP含量通過增加CC基因型，使IMP含量趨入合理。由F檢驗知:CASTRsa工酶切位點各基因型失水率值的差異顯著(p0.05)?？赏ㄟ^純合子EEFF雜交，增加EF基因型頻率來提高豬朋體持水能力。(2)雙酶切位點由F檢驗可知，肌脂含量值(%)在Hin月乙bl

5、sp工.WspIRsaI和llinfIlRsaI酶切位點，各基因型間肌脂含量值差異顯著(p0.05)，因此雙酶切位點可作為肌脂含量的遺傳標(biāo)記IlnfIRsaI酶切位點，各基因型間嫩度值差異顯著(p0.05)，因此，此酶切位點可作為嫩度的遺傳標(biāo)記。Abstract111AbstractPolymorphismofCASTgenewerestudiedusingRCRRFLPinfourswinebreeds:Duroc(50individ

6、uals)originRongchangpig(65individuals)Neijiangpig(38indivduals)andstrainIofNewRongchang(72individuals).TheassociationbetweenpolymorphismofCASTgeneandmeatqualitytraits(tendernessmeatcolourwaterfreelossinglossedwaterdripra

7、tionMarblingandintramuscularfat(IMP))wasanalysed.ThisstudyaimsatprovidingefectivegeneticmarkersforMASofmeatqualityandexploringtheresourcesofChinaspecialswinebreeds.Theresultswereasfollows:1Thegeneticvariationofthreeswine

8、breeds(1)TheCASTgeneofthreebreeds(DurocoriginRongchangNeijiangpig)weredigestedbyHinfID師IRsal.TheresultsshowedthattherewerepolymorphismsinthebreedsandatthislocusthepopulationswerenotatHardyweinbergdisequilibrium.(2)Theres

9、ultshowed:thedominantgenotypeofDurocNeijiangandRongchangwereABCCEFAACCEEAACCEFrespectively.(3)Therewasauncharacteristicbandsabout120bpbydigestedusingHinfinaDurocwhichnotfoundintheotherpigsatall.Theoccurenceofnewdigesteds

10、itemaybeattributedtotheenvironmentdiferencesofintroducingbreeds.2TheassociationbetweenpolymorphismsofCASTgeneofNewRongchangIandMeatqualitytraits.(1)TheproducedlocidigestedbyoneenzymesByFtesttherewereobviousdifferenceinma

11、rblingvalueswiththedifferentgenotypesdigestedbyHigJI(p0.05).ThusTheproducedlocidigestedbyHinfmaybeusedasgeneticmarkersofMarbling.therewereobviousdiferenceinIMPvalueswiththedifferentgenotypesdigestedbyMspI(p(0.05).ThusThe

12、producedlocidigestedbyMspImaybeusedasgeneticmarkersofIMP.WemayselecttheindividualswiththegenotypeofDDtoIMProvedaverageIMPvaluesinthepopulation.WemayalsoselecttheindividualswiththegenotypeofCCtomakeaverageIMPvaluesinthepo

13、pulationmorereasonable.therewereobviousdiferenceinwaterfreelossvalueswiththediferentgenotypesdigestedbyRsaI(p0.05).WemaycrosstheindividualswiththegenotypeofEEandFFtoImprovedtheabilityofsustainingwaterofbodyinthepopulatio