45354.erf類轉錄因子opbp1的功能研究

1、浙江大學博士學位論文ERF類轉錄因子OPBP1的功能研究姓名：陳旭君申請學位級別：博士專業(yè)：生物化學與分子生物學指導教師：郭澤建20060430浙江大學博士學位論文AbstractTheERFs(ethylell爭responsivcfactors)isuniquetoplants，containgaconservedDNAdomainRecently’itwasapproVedthattheexpressionofmanyERFgen

2、esisinvolvedinplalltdevelopment、met曲olism、paⅡ109eninfecaon，aswellasresponsetoetl、，iromentalsⅡ℃ss，suchashi曲salt、coId、出ou曲teta1RecentlyERFshavebeenevidcncedtoregulatedi仃IereIltplamsi弘alingp礎waysIthasbeeIlahottopictounderst

3、鋤dmeintemctionofdi插羽entplantsignalingpathwaysOsmotinpmmoterbindingproteinl(OPBPl)，wasis01atedusiIlgayeastonehybridsyst鋤RNAgelblota11dR1乙PCRanalysisindicatedmatexprcssionofthe0船PJgenewaSinducedbydicitorcryptogein，Nacl，efh

4、ephon，memylj踟onate，cycloheXimide，aswdlaspathogeIlattackItindicatemat0州妒Jisanimmcdiatce砌ygelle，粕dmaybeinvo】vedjnpJ柚tresponsetobiotic鋤dabioticstressTransi印texpressiona11alysisusinganoPBPleGFP如siongcneinonion印id咖alcellsreve

5、aledmatmeOPBPlproteinwastargetedtomenucle越Funher，elec咖phorcticmobili毋slliRassaysd鋤ons扛atcdmatmere60mbin枷OPBPlproteincouldbindtoaIloli90肌cleotidecontainingt11eGCCboxciselcmcntTransgeIlictobaccoplallts誦thanoVercxpressionof

6、the0朋PJg鋤eaccurnulatedh域levelsof瑚一』口and朋一尉genesande)￡llibi伽eIlhancercsist柚cetoin矗知onb了Pseudo∞n8ss：7ringnepvtnbnciandPhytophthor俚parnsntcq～aInicotinnnepathogensTheyalaoexhibitedincreasetolerancetosaltstrcssattheseedgerrni

7、natioIl，seedlingstageaSreflectedbymepercentageofgcⅡninatioll／孕ecnseedlings，the丘eshwei曲tofseedlings，andtheirdevcl叩mcntalpane兀1OntlleotherhaIld，tllea11tisense仃黜geniclines，whoseeXpressionof0P日尸Jgenewas訕ibited，bec啪emoresensi

8、tivetosaltstrcss姐dpathogenattackTr肌sgellicriceplantswimanoVercxprcssionofmeDP日PJgcneaccumulaledhigher1evelsofⅥfJ邑國M珊，蕊彤aand偽儺孤J，whichwerereportedtobeinvolvedinsaltt01eralltsi齟alingpamwayT11eoverexprssionrice1inesexhibite

9、denhaIlccdresistallcetoinfectionof肋t聊印Dr坼P∥毋g口，moretoleranttosalts訂cSsandoxidasesh℃ssTbus，oP8PJse鋤stobeanimportantdct鋤mantofbioticandabioHcs仃岱sresponscmplantsHowever，itwol】ldbeofintercsttohowWh曲erpalllog鋤resistallceands仃