27145.內(nèi)生固氮菌對環(huán)境因子的適應(yīng)性及其對多枝檉柳的抗旱效應(yīng)研究

1、新疆農(nóng)業(yè)大學(xué)碩士學(xué)位論文內(nèi)生固氮菌對環(huán)境因子的適應(yīng)性及其對多枝檉柳的抗旱效應(yīng)研究姓名：季華申請學(xué)位級別：碩士專業(yè)：生態(tài)學(xué)指導(dǎo)教師：羅明潘存德201006iiStudyofAdaptabilityofEndophyticDiazotrophicBacteriaStrainstotheDiverseEnviromentFactstotheDroughtResistanceofTamarixramosissimaLedeb.AbstractE

2、ndophyticnitrogenfixingbacteriawaslivinginnonleguminousplantsavoidingtheinhibitionofnitrogencompoundthecompetitionofindigenousmicrobialshowingahigherefficiencyofnitrogenfixation.Atthesametimeithasawiderangeofbiologicalec

3、ologicalroleofIAAProducingactivitysolubilizingphosphateactivityenhancingplantdiseaseresistancehasimptantapplicationvalueinimprovinghostnutritioncompetitivenessenhancingthehostofbioticabioticstressresistancepromotingtheab

4、ilitytoadapttheirenvironment.Studyofadaptabilityof5endophyticdiazotrophicbacteriastrainsisolatedfromtherootofTamarixramosissimaLedeb.LyciumruthenicumMurr.atthelowerreachesofTarimRivertothediverseenviromentfacts.Inthisstu

5、dyChoosethestrainDENB20hadsuperitolerancetostressofthediverseenviromentfacts.ColonizingdynamicofthestraininrootofT.ramosissimaunderdroughtstresstheeffectsofendophyticbacteriaonthedefenseenzymesactivities.Themainresultssh

6、owed:ThestrainDENB2DENB6DENB19DENB20DENB55couldgrowexhibitnitrogenaseactivityunderwiderangeoftemperaturefrom1540℃pHfrom5.010.0.Thestrainscouldalsotoleratetosodiumchlideconcentrationsof0.1%5.0%NH4concentrationsof0.54mmolL

7、1arangeof－0.30.15mPaosmoticpotentialrespectively.Itwassuggestedthatthestrainshadsuperitolerancetostressofthediverseenviromentfactsbuttheirenduranceabilitiesdifferpartly.ThestressresistanceofstrainDENB20isthebest.Thestrai

8、nDENB20withthemutagentreatmentobtainthestrainDENB20rscouldresisttheconcentrationof150gmL1rifampicinstreptomycin.ThestraincouldcolonizationintheL.ruthenicum.Underdroughtstressitsdensitydecreasedinleafthestraincouldn’tisol

9、atedfromleaveswhenwatercontrolto20dwiththegrowthofseedlingitsdensityincreasedinrootthehighersoilmoisturecontentthemethenumberofbacteria.Viewfromthesoilmoistureconditionsthedifferencesignificantofcolonizingdynamicsofthest

10、rainunderwatercontrolbefeafter.Thestraincaninprovetheseedgerminationrateunderdroughtstresscansignificantlyimprovetheplantbiologicalnitrogenfixationincreasethenitrogencontentofplant.Improvingplanttoabsbutilizethepotassium

11、phosphus.Changesinsoilmineralnutrientinparticularsoilganicmattercontentthuspromotingplantgrowthincreasingdiseaseresistance.Astheintensityofdroughtstressstresstimethehigherdryfreshweightsofendophyteinfectedplantswerehighe

12、rthanthoseofendophytefreeplants.ThecontentofchlophyllactivitiesofSODPODCATRootactivityofendophyteinfectedplantswerehigherthanthoseofendophytefreeplants.TheactivitiesofMDAProcontentsinendophytefreeplantswerehigherthanthos

13、eofendophyteinfected.Itshowedthatthestraincanpromotetheabilityofdroughtstressofplant.ThestrainDENB20growwellinacidicconditionstheutilizationofinganicnitrogenwashigherthanganicnitrogensources.WithMaliacidascarbonsource30℃