幾種毛皮動物病毒性傳染病病原核酸及血清抗體的檢測.pdf

1、東北林業(yè)大學碩士學位論文幾種毛皮動物病毒性傳染病病原核酸及血清抗體的檢測姓名：王玉龍申請學位級別：碩士專業(yè)：野生動植物保護與利用指導教師：華育平張洪英20040601AbstractCanineDistemperandCanineParvovirusEnteritisare垃瑤twomostimportantviralinfectionscommonlyoccuramongfarmedfuranimals，whileFoxEncepha

2、litisisakindofviralinfectionamongfoxesIthasbeendecadessincetheprevalenceofthesethreekindsofinfectionsamongfuranimalsTheyhaveoccurredinalmosteveryraisingareaandfarmthroughouthistoryAllofthesethreeinfectionsOCCUranddevelop

3、atahi秘speedOncetheyprevail，itwillbefollowedbyhighincidencerateandmortalityAgreatnumberofdeathsCannotonlyleadtosevereeconomiclossfortheowners，butalsobadlyinfluenceandrestrictthedevelopmentofthewholeindustryRecentlythesein

4、fectionsa辯wellpreventedandbettercontrolledthanbefore，however，theystilloccllrandprevailever)yearbecauseofvariousofreasonsInordertopreventandcontroltheseinfections，efficientsimple，rapidreliableandspecificdiagnosismethodsar

5、erequiredMoreoverweshouldcomprehendtheoccurrenceandprevalenttrendbeforehandInthisstudywe(1)sampledroutinevaccinatedanimalsfromsomefarmswhichraise：morethan3000furanimalsinShahDongandHeiLon螽inangprovincesoct(2)optimizedthe

6、PCRprotocolfordetectionanddiagnosisandatthesametimegivespecificdetectiononnucleicacidinCDVandFoxEncephalitisvirusfromsamplescollectedfromvaccinatedanimals8)appliedimmunoenzymaticassaytodetectandanalyzeSCIXUlIantibodiesti

7、terinCDVandFoxEncephalitisvirus；whileapplyingHAandHItodetectandanalyzeserumantibodiestiterinCPV(4)arlalyzedantibodylevels，comprehendeffectsofimmunityandanalyzeleasonsoffailureforimmuni≮Connectingprevalentconditionsinrece