殺蟲(chóng)劑對(duì)松墨天牛幾種酶和蛋白質(zhì)磷酸化的影響.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)碩士學(xué)位論文殺蟲(chóng)劑對(duì)松墨天牛幾種酶和蛋白質(zhì)磷酸化的影響姓名：劉杰申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：農(nóng)業(yè)昆蟲(chóng)與害蟲(chóng)防治指導(dǎo)教師：高希武伍一軍20050601AbstractMonochamusalternatusHope(Coeoptera：Cerambycdae)isnotonlyaseriouspesttoPinusbutalsothemainvectorofpinewoodnemadoteBursaphelenehusxyloph

2、ilus，whichcausespinewiltdiseaseThere’smanyresearchesonthisinsectaboutitsbiology，ecology，andthemethodologyofitscontr01Inthisthesis，methamidophos，deltamethrine，andimidaclopridwerechosentobetherepresentativesofthreekindsofi

3、nsecticidestotestitseffectonacetyIcholinesterase(ACHE)，gtutathionestransferase(GSD，andcarboxylesterases(carE)TheauthorstudiedtheeffectofmethamidophosanddeltamethrineOilproteinphosphorylationofthisinsectusingradioactiveis

4、otopelabelingmethodInadditionweassayedactivityofneurotoxicesterase州TE)inthispestinsect，andtriedtodoprimarycultureofthehemocytesandfatbodycellsfromthisinsectThedetailsoftheresultsareasfollows：Methamidophoscouldinhibitnoto

5、nlyAChE，andCarEbutalsoGSTinvitro；the150valuesoftheinhibitionconcentrationsare819Xi0一M，19510。。M，andO908MforthethreeenzymesrespectivelyItiscertainthatthereisNTEactivityinMonochamusalternatusTheactivityoftheheadoftheinsecti

6、shigherthanthatofotherparts【thasnotbeenobservedthatmethamidophoscaninhibitN1’Eactivityinvitro，whichisquitedifferentfromthesituationfoundinvertebratesMethamidophosanddeltamethrinhavesignificanteffectonthephosphorylationof

7、proteinsfromMonochamusaltematuslarva；howeveLtheeffectofthetwoinsecticidesontheproteinphosphorylationoftheadultislesssignificantthanthatofthelarvaDeitamethrinenhancethephosphorylationoftheproteinswithmolecularwei【ghtof137

8、8kDand1665kDfromfatbodyandheadoftheinsectlarvarespectivelyItinhibitsthephosphorylationofproteinsofllI4kD，953kDand438kDfromlarvafatbody1132kD，975kD，50，6kDfromlarvahead518kD，443kDfromadultfatbodyand433kDfromadultheadMetham

9、idophosenhancethephosphorylationleveloftheproteinswithmolecularweightof1277kD，443kD，and2389kDfromadultfatbodybutitinhibitthephosphorylationofproteinsof953kDand438kDfromlarvafatbodyand1665kDandl245kDproteinfromlarvaheadWe

10、acquiredmanyinformationoftheproteinkinaserelatedwithproteindetectedbyautoradiographythroughusingcAMPandcalmodulinintheassaysystemBecauseofthecomplicacyoftheproteinphosphorylationsystemfurtherresearchworkisneededtogainmor

11、einformationabouttheproteinkinaseandproteinphosphataseTheresultofourresearchwillbebenificialtothecontrolofMnonchamzlsalternatusTheeffectsofmethamidophosanddeltamethrinoRproteinphosphorylationindicatedthatthetwoinsecticid

12、esmayhavemoreothertargetsthanthefataltargetswhichcouldkillinsectinshorttimeKeywords：Monochamusalternatusinsecticideacetylcholinesterase(ACHE)glutathionestransferase(GST)carbexylesterases(CarE)proteinphosphorylationprimar