Math1-NT3腺病毒的構(gòu)建及其在體共轉(zhuǎn)染C57BL-6J老年小鼠耳蝸的初步研究.pdf

1、東南大學(xué)碩士學(xué)位論文Math1NT3腺病毒的構(gòu)建及其在體共轉(zhuǎn)染C57BL6J老年小鼠耳蝸的初步研究姓名：徐琳申請學(xué)位級別：碩士專業(yè)：耳鼻咽喉科指導(dǎo)教師：高下高翔20080608英文摘要AbstractSensorycelllossresultingfromaging，ototoxicdrugs≈infections，overstimulationandothercausesisirreversibleandleadstopermanen

2、tsensorineuralhearinglossinthemammalianauditorysystemItisnecessarytogeneratenewfunctionalhaircellstestorehearingMathl，thebasichelix—loop—helixtranscriptionfactor，isapositiveregulatorofhaircelldifferentiationduringcochlea

3、rdevelopmentDevelopinghaircellsexpressMathl，andnonsensorycellsdonotNT3isamemberofthefamilyofgrowthfactorswhichhasitsmRNAhighlypresentedinspiralganglionneuronsAvarietyofstudiesinvivoandinvitrosuggestNT3tobetheprimarysurvi

4、valfactorsfortheinnerearandlikelyapotentialtherapeuticagentforsensorineuraldeafnessInourstudyweraisedahypothesisthatthecombinationdeliveryofMathl／NT3geneintocochleaCallpromotehaircellsregenerationandspiralganglionneurons

5、survivalHoweverdissectionspecificity，suchasrelativelyhermetizationandindependentandthepresenseofbloodlabyrinthbarriermakeitdifficulttodeliverexogenousmaterialsintocochleaSoitwillbeagreatachievementtostudytheeffectofMathl

6、／NT3genedeliveryintocochleatoguidetheclinicaltherapyforsensorineuraldeafnessInthisstudy，22一month—oldC57BL／6JmicewereusedasmodelsforpresbycusisThegenetherapyprocedurewithincochleawereestablishedsuccessfullyByimmunohistoch

7、emistry，histopathologyultrastructuralpathologytechniques，weobservedthemorphologicalchangesafteradenovirusinjectiontoevaluatetheeffectsofcombinationdeliveryofMathl／NT3intocochleaanditspotentialtopreserveasawaytoprovesenso