藏雞胚胎低氧適應(yīng)的血紅蛋白分子機(jī)制.pdf

1、、，773651分類號：密級：’j目差嚏夭等學(xué)位論文單位代碼：10019學(xué)號：B02153藏雞胚胎低氧適應(yīng)的血紅蛋白分子機(jī)制HemoglobinMechanismofHypoxiaTolerationinTibetanChickEmbryo研究生：苞遺指導(dǎo)教師：縣堂籃數(shù)援一合作指導(dǎo)教師：整鹽生熬撞至主塾援申請學(xué)位門類級別：農(nóng)學(xué)博士專業(yè)名稱：動塑塑籃直抽皇繁殖研究方向：麴塹坌王鏨量速佳堂所在學(xué)院：動塹型堂撞本堂暄——二00五年六月中國農(nóng)業(yè)

2、大學(xué)博士學(xué)位論文AbstractAbstractTibetanchickacclimatizethemselvesuptohighaltitude(4，200m)andtheotherbreedsinlowgroundssurviverarelywithalowhatchability(3O％)afterincubationunderhypoxiaofsimulated4，200mInhypoxiaofwhole21daystheinc

3、ubationrateofTibetanchickandRecessiveWhiteFeatherbroilerdifferedwithagreatestdisparityfromthe4thtothe11thdayandsignificantlyinotherstagesbutfrom1stto3rddayHypoxiaineachstagedidnotreducesignificantlysurvivalrateofthisstag

4、eexcepthatchabilityThesetworesultsindicatedthatthehypoxiainlaststagehaveakeyeffectonnextstageAllexonsencodingchickhemoglobinsweresequencedtoanalyzegenepolymorphismThefunctionalmutationofMet一32(B13)Leufoundin—globinchainw

5、asrelatedwithhypoxiaandthemutationfrequencyincreasedwithelevatedaltitudesInaddition，thepopulationwithhighermutationfrequencyhadahigherhatchabilityTheautomatedhomologymodelbuildingwascarriedoutusingcrystalstructurecoordin

6、atesofchickHbD111eresultsindicatedthatthesubstitutionofMet32(Bl3、LeuprovidesamolehydrophobicsurroundingwhichleadtohigherstabilityofheineandbloodoxygenaffinityofhemoglobinTheincubationunder21dayhypoxiaf12％02)wascarriedout

7、inTibetanchickfromhighlandandRecessiveWhiteFeatherBroilerfromjowlandThewholeembryosfrom3rdto8thdayandwholebloodfrom9thto18thdaywascollectedtoinvestigatedthestageexpressionprofilesofwholesevenglobinsandHIF10Theresultsindi

8、catethat，岫derhypoxiathemajoradultffAand13globinsbecomemajorfrom3rdto8thdaywhilemajorembryonicEandPglobinsbecomeminorandminorembryonic￡globinbecomemajorinbothbleeds；Theminor13“and0tnglobinsalsoserveasthemajorfetalglobinsi

9、ntheembryofrom9thto18thdayunderhypoxiainbothbreedsTheexcessiveexpressioninducedbyhypoxian2％02)，notproducedbyearlierexpressionofHbA，HbHandHbL，isfoundasthemostimportanthemoglobinmechanismofhypoxiaadaptationandisuniquetoTib

10、etanchickThemutationMet32D(B13)LeuactasanimpoOanteffectonhypoxictolerationafterday9andinadultstage，insteadofdays38Days38isMlostimportantstageforsurvivalunderhypoxiccondition，whichisconsistentwiththeresultofstageincubatio

11、nandexpressionofHIF—la。TheoverexpressedHIFIashouldberesponsibletotheearlierexchangeoftheprimitiveanddefinitiveerythoidcells，theswitchofembryonicandadultglobinsandtheexcessiveexpressionofallglobinsinTibetanchickbydirectiv

12、elyregulatingsuchfactorsasEPO，GATA一1，GATA一2，SPl，Y，NFIandNFE4、一ThemutationMet32D(B13)一LeuhasbeendescribedforthefirsttimeinhighlandpheasantThelowlandred—ringpheasantandhighlandpheasantbelongtodifferentsubspecies，thusthesam