南方主要豇豆和花椰菜品種的鑒定研究.pdf

1、Y900‘3洳≥：j、法碩士學(xué)位論文⑧南方主要豇豆和花椰菜品種的鑒定研究作者姓名選睦賢指導(dǎo)教師朔置熬援學(xué)科(專業(yè))佳塹遺佳直弛所在學(xué)院壅!叢直生蛔撞盔堂隍提交日期三魚魚盎生垂旦TheidentificationofmaincowpeaandcauliflowervarietiesinsouthofChinaAbstraetVarietalidentificationisimpor[anttoensuretheseedqualityInt

2、hepresentstudyefficientidentificationnaeflaodsforthemainvarietiesofcowpea(慟口unguiculataw)andcauliflower(BrassicaoleraceaL)insouthofChina，especiallyinzhejiangprovince，wereinvestigatedbyusingsevencowpeavarieties(varietyl，Z

3、hijiangtechangS0；variety2，Zhijiangtechan990；variety3Qiujian9512；variety4，Zhijian9228—2；variety5，Huajian924；variety6，Yangjian940；andvariety7Xingaochan8)andfivecauliflowervarieties(varietyA，Longfenteda50tian；varietyBYida巧i

4、nguangS0tian；varietyC，Ruixuetedal00tian；varietyD，QiuwangS0tian；andvarietyE，Teza050tian)Theapplicationofseedshape，seedlingshape，rapidchemicalidentificationandelectrophoresiswasperformed，themainresultswereasfollowsTheresul

5、tsshowedthattherewasnodifferenceinseedcolorandlusteramongsevencowpeavarieties，theseedlength／thickratiooffivevarietieswassignificantdifference，theseedlength／widthratioofonevarietywasidentifiedfromtheothers，SOthesixvarieti

6、escouldbeidentifiedbyseedshapeThevariety3couldbeidentifiedbyphenolandguaiacoltest，theothervarietiesalsocouldbeclassifiedintothreegroupsThevariety4and5couldbedistinguishedbylengttVwidthratioofseedlingleavesInthepresentstu

7、dythelacticacidtankbuffer(containing15％sucroseand005％methylgreen)wasselectedasextractionsolution，andseedswereputinwaterpotfor05hat606CforproteinextractionBythismethod，theelectrophoregramsgaveabundancebanddiversityandcoul

8、didentifiedvarietyl，3and5TherewasalittledifferenceinthecolourofseedcoatandembryoofcauliflowerbutitwasnotobvioustoidentifyvarietiesThevarietyA，varietiesBandE，varietiesCandDcouldbedistinguishedby10％NaOHsolutiontreatment，th

9、evarietyBalsocouldbeidentifiedbyHCIandguaiacoltestByusingtheseedlingshapeidentification，thevarietyCwithpurplecolourwasdentifiedfromCheothervarietiestherestofvarietiescouldbeclassifiedintotwogroups：varietyAandB，varietyDan