花椰菜核型分析及單條染色體的顯微分離.pdf

1、福建農(nóng)林大學碩士學位論文花椰菜核型分析及單條染色體的顯微分離姓名：夏法剛申請學位級別：碩士專業(yè)：蔬菜學指導教師：周元昌何承坤2002.5.1壟豎苤鱉型坌塹墨苧墨墨魚堡塑墨垡坌塞一一————————————————三AbstractcaulinowetfBrassicaoleraceavarbotrytisL)isoneofthemostimportantvegetablesItisverypracticallymeaningfultos

2、tudykaryotypeandtomicrodissectsinglechromosomeofcauliflowerforestablishingitsDNAlibraryandgeneticmapaswellasclo咖gitskeygenesInthispaperthecauliflowervariety“Fuzhou60day”，wasusedasexperimentalmaterialforfollowingstudies1T

3、heexperimentalconditionsofkaryo卯eanalysisandmiscrodisscefionwereoptimizedTheresultswereshownasfollows：(1)Verygoodchromosomesampleswereobtainedbymacerating60。C1NHelfor10minutes，andsimilareffectcouldalsobeobtainedbymacerat

4、ing1NHClfor12—16minutesatroomtemperaturer2)WhentherootlengthwasaboutO5cmwasthebesttimetopretreat(3)GoodshapeofchromosomescouldbeobtainedandthechromosomeswereeasytObeidentifiedbypretreatingwith8Hydroxyquinoline，旺一Bromonap

5、hthalene，cycloheximideandpDichlorobenzeneAndpretreatmentwith8Hydroxyquinolinecouldreachthebestresult，namelychromosomeswerefairlywelldispersed，theprimaryandsecondaryconstrictionsweredistinctandthechromosomeswithsatellitew

6、ereeasytobeidentified(4)Formicrodissection，thechromosomesampleshould如rtherbemaceratedwithasolutioncontaining2％cellulaseand2％pectinaseat37。Cfor1～15hourwhichcouldrefrainfromthebackgroundinterfereuceofcytoplasmandcellwall2T

7、heanalysisofkaryot)甲eof“Fuzhou60day”showedthat：thesomaticchromosomenumberswere2n=l8，thechromosomewithsatellitewaslocatedinchromosome7withaalinratio201Thelengthofthechromosomeswasbetween205and3909intherelativelengthWasbet