豇豆胰蛋白酶抑制劑（CpTI）基因轉(zhuǎn)化花椰菜的研究.pdf

1、西南農(nóng)業(yè)大學(xué)碩士學(xué)位論文豇豆胰蛋白酶抑制劑（CpTI）基因轉(zhuǎn)化花椰菜的研究姓名：呂玲玲申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：雷建軍宋明2003.5.1StudyonTransformationofCowpeaTrypsinInhibitorgeneintoCauliflower(BrassicaoleraceaVHFbotrytis)AbstractTherearesomeresearchesabouttransferringBtge

2、neintocau]i—flower，bucfewwerereportedabouttransferringCpTIgeneintocauli—flowerInthispaper，cotyledonsandhypocotylswereusedasexplants，bymeansofplantgeneticengineering，foreigninsect—resistancegenecanbetransferredintoplantce

3、llCopeatrypsininhibitor(CpTI)genewastransferredintocauliflowerbyagrobacterium—mediatedtransformationmethodandthetransgeniccauliflowerplantswereobtainedThemainresultsareasbelow：1Highefficiencyregenerationsystemofcauliflow

4、erThehighefficiencyregenerationsystemisthebaseofplantgenetictransformationTheexplantswerecotyledonsandhypocotylsandMSwasusedasbasicmidiumBysupplyingdifferentconcentrationof6一BA，NAA，wegottheoptimummediawhichinducedtheadve

5、ntitiousbuddifferentiationoftwocauliflowerexplantstypesTheoptimummediaisMSBA2Omg／LNAAO2mg／LBetweenthetwoexplantstypes，thehypocoty]sexpressedthehigheradventitiousbuddifferentiationcapacity2ThebestKanconcentrationusedtosel

6、ectthetransformantplantsExplantswererestrainedfromdifferentiatingwhenKanwasaddedtothemedium，moreover，somecallusturnedwhitegraduallyanddiedWhenKanconcentrationwashigherthan15mg／L，alloftheexplantsandcallusbecamedeadSothebe

7、stselectiveconcenbrationofKanis15mg／L3TransformationsystemofcauliflowerTheproceduresdescribedwerederivedfromnumerousregenerationandtransformationdesignedtotestfactorsthatmightaffectshootregeneration7restedparametersinclu

8、delengthofprecultere(2d，3d)，lengthofinoculationwithagrobacterium(30—60sec，5lOmin)，lengthofcocultivation(1d，2d，3d)，Kancocentration(0，5，lO，15，30，50mg／[)On]Ythoseparametersproducingthebestresultaredescribedasbe]OW：Thecanlif